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| 人参皂苷Rb1抑制AngⅡ诱导的心肌细胞肥大 | |
| 引用本文： | 陈小文,黄燮南,吴芹. 人参皂苷Rb1抑制AngⅡ诱导的心肌细胞肥大[J]. 遵义医学院学报, 2008, 31(5) |
| 作者姓名： | 陈小文 黄燮南 吴芹 |
| 作者单位： | 遵义医学院,药理学教研室,贵州,遵义,563003 |
| 摘 要： | 目的观察人参皂苷Rb1(ginsenoside Rb1,Rb1)对血管紧张素Ⅱ(angiotensin Ⅱ,AngⅡ)所致心肌细胞肥大的影响。方法利用培养的新生大鼠心肌细胞,以细胞直径、蛋白含量为心肌肥大标志,观察药物的抗心肌肥大效应。ANFmRNA的表达用Real-timePCR检测;用Fura-2/AM负载的培养心肌细胞检测细胞内游离钙浓度([Ca2+]i)以探讨Rb1可能的作用机制。结果AngⅡ1×10-7mol.L-1使心肌细胞细胞直径明显增大,蛋白含量明显增加,心房利钠因子(atrial natriuretic factor,ANF)mRNA表达明显上调,并使细胞[Ca2+]i明显升高。Rb150、100和200μg/ml使经AngⅡ处理的心肌细胞直径分别缩短18.4%,32.7%和43.8%;心肌细胞蛋白含量分别减少8.4%、13.6%和17.2%;降低ANFmRNA的表达;Rb150、100和200μg/ml呈剂量依赖地抑制AngⅡ所致的[Ca2+]i升高,NO前体L-精氨酸L-arg10-3molμL-1具有相似的作用,NO合酶抑制剂L-NAME对Rb1的效应无明显影响。结论Rb1可抑制Ang... |
| 关 键 词： | 心肌细胞肥大 血管紧张素Ⅱ 人参皂苷Rb1 细胞内游离Ca~(2+)浓度 |
Ginsenoside Rb1 inhabits cardiomyocyte hypertrophy induced by angiotensinⅡ |
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| CHEN Xiao-wen,HUANG Xie-nan,WU qin. Ginsenoside Rb1 inhabits cardiomyocyte hypertrophy induced by angiotensinⅡ[J]. Acta Academiae Medicine Zunyi, 2008, 31(5) | |
| Authors: | CHEN Xiao-wen HUANG Xie-nan WU qin |
| Affiliation: | CHEN Xiao-wen,HUANG Xie-nan,WU qin(Dept of pharmacology,Zunyi Medicai College,Zunyi Guizhou 563003,China) |
| Abstract: | Objective To study the effects of ginsenoside Rbl(Rbl) on cardio myocyte hypertrophy induced by angiotensin Ⅱ(AngⅡ),and to probe primarily its mechanism.Methods The cultured neonatal rat cardiomyocyte hypertrophic response induced by Ang Ⅱ and the antihypertrophic effects of Rbl were observed by measuring the cell diameter and protein content.the expression of atrial natriuretic factor(ANF) mRNA,which was assayed by real-time PCR.For mechanism study,the intracellular free calcium concentration([Ca2+]i) in c... |
| Keywords: | cardiac hypertrophy angiotensin Ⅱ ginsenoside Rb1 cardiomyocyte |
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