水溶性BODIPY类近红外荧光探针的合成及生物组织成像研究

目的 长波吸收和发射的水溶性荧光染料被广泛应用于生物技术和生物医学领域,如DNA排序、诊断成像和光动力治疗等.方法 为了开发具有近红外荧光特性的分子探针,通过Knoevenagel缩合反应合成一种新型水溶性荧光化合物——荧光染料(BODIPY);评价了其光学性能,并以裸鼠为实验动物评价了该探针在生物组织成像中的效果.结果 这类荧光化合物具有良好的水溶性和光学性能,对正常裸鼠深层组织和脏器具有明显成像效果.结论 该类探针在荧光影像学诊断方面具有很好的应用潜力.

Synthesis and biotissue imaging of water-soluble boron-dipyrromethene fluorophore

Objective Water-soluble fluorescent organic dyes with absorbing and emitting in the relatively long wavelength range （around or beyond 600 nm） can be used for biotechnology and biomedical applications such as DNA sequencing, diagnostic imaging, and photodynamic therapy. Methods A novel water-soluble boron- dipyrromethene （BODIPY） fluorophore was synthesized by Knoevenagel condensation reaction, and evaluated the optical performance. In vivo fluorescence imaging effect and organ distribution of the fluorophore was conducted with normal nude mice. Results The fluorophore had good water-solubility, strong emission ability in long wavelength range and high fluorescent quantum yield, result showed that the fluorophore have obvious imaging effect in deep tissues and whole organisms. Conclusion The near-infrared fluorescent probes have many potentials in applications in the medical imaging.