冠心巴布膏活血化瘀作用研究

目的:探讨冠心巴布膏(冠心膏)的活血化瘀作用,为其临床应用提供实验依据.方法:采用大鼠sc肾上腺素加冰浴的血瘀证模型,观察冠心膏高、中、低3个剂量(含生药10.0,5.0,2.5 g·kg-1)外用涂抹给药5d对模型动物全血黏度、血浆黏度、纤维蛋白原含量、红细胞压积、血小板聚集的影响.采用iv脑垂体后叶素(0.5 U·kg-1)造成大鼠急性心肌缺血模型,观察冠心膏(剂量同前)外用涂抹给药7d对模型动物心律失常的发生时间、持续时间及室性心律失常发生率的影响.结果:冠心膏高剂量能降低血浆黏度[由(1.101±0.064) mPa·s降至(1.039±0.025) mPa·s]、血小板聚集率[由(54.92±6.53)％降至(49.05±4.15)％]、200 s-1切变率下的全血黏度[由(3.81±0.31) mPa·s降至(3.53±0.27) mPa·s],延迟心律失常的开始时间[由(20.90±7.74)s升至(36.08±16.91)s],并降低心律失常的发生率(由75％降至37.5％)及持续时间[由(1133.75±298.71)s降至(881.20±115.48)s].低剂量能降低10 s-1切变率下的全血黏度[由(7.14 ±0.88) mPa·s降至(6.38±0.69) mPa·s]以及200 s -1切变率下的全血黏度[由(3.81±0.31) mPa·s降至(3.48±0.15) mPa·s],中剂量可以降低心律失常发生率(由75％降至37.5％)和持续时间[由(1133.75±298.71)s降至(816.43±243.46)s],与模型组比较均具有显著性差异(P＜0.05,P＜0.01).结论:冠心巴布膏可以改善血瘀证大鼠的血液流变学指标,防止心律失常的发生,具有一定的活血化瘀作用.

Study of Promoting Blood Circulation for Removing Blood Stasis by Guanxin Cataplasm

Objective:To investigate the actions of promoting blood circulation for removing blood stasis by Guanxin Cataplasm and provide the experimental basis for clinical applications of the cataplasm.Method: The rat blood stasis syndrome model was induced by subcutaneous injection of adrenalin with ice-bath,the indexes of hemorheology including fibrinogen,whole blood viscosity,plasma viscosity,packed cell volume and platelet aggregation.The acute rat myocardial ischemia model was established by sublingual vein injection of 0.5 U·kg-1 posterior pituitary,and the electrocardiogram ECGs were recorded by polygraph to determine the incidence of ventricular tachycardia(VT) and ventricular fibrillation(VF),the mean duration and onset time of VT plus VF.Result: Compared with model group,high dosage group of guanxin cataplasm decreased plasma viscosity from(1.101±0.064) mPa·s to(1.039±0.025) mPa·s,platelet aggregation from(54.92±6.53)% to(49.05±4.15)%,and whole blood viscosity under the shear rate of 200 s-1from(3.81±0.31) mPa·s to(3.53±0.27) mPa·s;meanwhile the delayed duration of VT plus VF from(1 133.75±298.71)s to(881.20±115.48)s and onset time of VT plus VF from(20.90±7.74)s up to(36.08±16.91)s with reduced incidence of VT plus VF from 75% to 37.5% were observed.Small and middle dosage group led to a reduction in whole blood viscosity under 10 s-1 from(7.14±0.88) mPa·s to(6.38±0.69) mPa·s and that under 200 s-1 from(3.81±0.31) mPa·s to(3.48±0.15) mPa·s shear rate,and in the incidence decreased from 75% to 37.5% and duration decreased from(1 133.75±298.71) s to(816.43±243.46) s for VT plus VF respectively(P<0.05,P<0.01).Conclusion:Guanxin cataplasm can obviously improve indexes of hemorheology and afford a protection against occurring of VT plus VF,which may contribute to its function of promoting blood circulation for removing blood stasis.