益气养阴化浊通络方对早期糖尿病肾病患者 CTGF,IV型胶原及Cys C表达的影响

目的: 探讨益气养阴化浊通络方对早期糖尿病肾病(DN)患者血清结缔组织生长因子(CTGF)、IV型胶原及胱抑素(Cys C)表达水平的影响。 方法: 63例早期 DN 患者随机分组,对照组(31例)采用DN常规治疗,治疗组(32 例)在常规治疗基础上加用益气养阴化浊通络方,疗程2个月。采用酶联免疫吸附法(ELISA)测定两组患者治疗前后血清CTGF,IV型胶原蛋白含量,颗粒增强透射免疫比浊法(PETIA)测定Cys C水平,同时观察空腹血糖、尿微量白蛋白排泄率(UAER)的变化。 结果: 与治疗前比较,2组患者治疗后CTGF,IV型胶原水平下降(P＜0.05,P＜0.01), Cys C,UAER,空腹血糖指标改善(P＜0.05,P＜0.01)。同时治疗组治疗后CTGF,IV型胶原,Cys C水平改善优于对照组(P＜0.05,P＜0.01)。 结论: 益气养阴化浊通络方可通过降低DN 患者CTGF,IV型胶原水平而延缓 DN 的发展。

Effect of Yiqi Yangyin Huazhuo Tongluo Recipe on Plasma CTGF, Type IV Collagen and Cys C in Patients with Early Diabetic Nephropathy

Objective:To investigate the effect of Yiqi Yangyin Huazhuo Tongluo recipe(YQYYHZTLr) on plasma connective tissue growth factor(CTGF),type IV collagen and Cystatin C(Cys C) in patients with early diabetic nephropathy(DN).Method: Six-three patients with DN were randomly divided into the control group(n=31) and the treatment group(n=32).The control group was applied DN regular treatment and the treatment group was added to YQYYHZTLr on the basis of regular treatment.Both of them were treated for two months.Plasma CTGF and type IV collagen were determined with ELISA in two groups both before and after treatment.Plasma Cys C was determined with particle-enhanced turbidimetric immunoassay(PETIA).Fasting blood glucose and urinary albumin excretion rate(UAER) were also examined.Result: Plasma levels of CTGF and type IV collagen between the two groups were significantly lower than those of pretreatment(P<0.05,P<0.01),and favorable changes of levels of other parameters(Cys C,UAER and fasting blood glucose) were also demonstrated(P<0.05,P<0.01).At the same time,plasma levels of CTGF,type IV collagen and Cys C were different significantly in the treatment group after treatment compared with the control group(P<0.05,P<0.01).Conclusion: YQYYHZTLr could delay the development of early DN by decreasing the levels of CTGF and type IV collagen.