圣愈超微粉颗粒改善气虚血瘀型高脂血症大鼠血管内皮功能和抗氧化作用

目的:观察圣愈超微粉颗粒对气虚血瘀型高脂血症大鼠血管内皮功能及抗氧化作用的影响.方法:以游泳过劳加高脂饲料喂养30 d,制备气虚血瘀型高脂血症大鼠模型；分正常、模型、阳性药通脉颗粒组(2.4 g·kg-1)、圣愈超微粉颗粒高、低剂量组(3.2,2.4 g·kg-1),均ig 3周；测定血脂4项总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(HDL-C)和高密度脂蛋白胆固醇(LDL-C),血清一氧化氮(N0)、血浆内皮素(ET-1)；血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的水平.结果:与正常组比较,模型组大鼠血清TC,TG,LDL-C,MDA,血浆ET-1水平均升高(P＜0.05或P＜0.01),血清NO水平降低(P＜0.05),血清SOD及GSH-Px水平显著降低(P＜0.01)；与模型组比较,通脉颗粒组、圣愈超微粉颗粒高剂量组TC,TG,LDL-C,MDA,血浆ET-1水平均明显降低,高剂量组分别为(2.03±0.23),(1.44±0.30),(0.98±0.17)mmol·L-1],(6.92±1.39)mol·L-1,(143.39±17.33)ng·L-1；模型组分别为(3.09±0.23),(2.05±0.15),( 1.48±0.15)mmol·L-1],(8.59±1.541) mol·L-1,(163.13±33.61)ng·L-1)(P ＜0.05或P＜0.01),血清SOD,GSH-Px和NO水平升高,高剂量组分别为(229.39±38.333),(979.44±197.453) U· mL-1],( 34.92±12.39)μmol·L-1；模型组分别为 (163.13±33.61),(513.49±122.60) U·mL-1],(23.39±8.54) μmol·L-1(P ＜0.05或P＜0.01)；圣愈超微粉颗粒低剂量组作用相对较弱,仅能够显著下调血清TC,SOD和GSH-Px水平.结论:圣愈超微粉颗粒与阳性药通脉颗粒相似,具有明显的改善气虚血瘀型高脂血症大鼠血管内皮功能及抗氧化作用.

Effect of Shengyu Superfine Powder Granules on Vascular Endothelium and Antioxidant Action in Hyperlipidemia Rats with Qi Deficiency and Blood Stasis

Objective: To investigate the effects of Shengyu superfine powder granules on protection to vascular endothelium and antioxidant in hyperlipidemia rat in qi deficiency and blood stasis. Method: The rat model was experimentally induced by feeding with high glucose and fat diet together with swimming overfatigue. Wistar rats were randomly divided into the control group, the model group, the positive group and the Shengyu superfine powder granules groups (high doses and low doses). After feeding 3 weeks, the positive group was given Tongmai Granules(2.4 g·kg^-1) and different doses of Shengyu superfine powder granules(3.2 or 1.6 g·kg^-1) were fed to the rats. Then the levels of total cholesterol(TC),triglyceride(TG), high density lipoprotein cholesteroal(HDL-C), low density lipoprotein cholesterol(LDL-C), nitric oxide(NO), endothelin-1(ET-1), malondialdehyde(MDA), supevoside dismutase(SOD), glutathione peroxidase(GSH-Px) were assayed. Result: Compared with the normal group, the level of NO,SOD,GSH-Px of the model group was decreased, the level of TC,TG, LDL-C,ET-1, MDA was increased. Compared with the model group, the levels of NO,SOD,GSH-Px of the positive group and the Shengyu Superfine powder Granules(high dose) group were increased obviously, the level of TC,TG, LDL-C, MDA was decreased obviously, the level of ET-1 of the positive group was decreased obviously. Compared with the positive group, the levels of TG, LDL-C of the Shengyu superfine powder granules(low dose) group were increased obviously, the levesl of NO, SOD, GSH-Px were down-regulated. Conclusion: Shengyu superfine powder granules could provide protection on vascular endothelium as well as antioxidant action in the rats with hyperlipidemia.