阿霉素热化疗对肝癌细胞增殖和凋亡的影响

目的:探讨阿霉素(ADM)加热化疗对人肝癌细胞HHCC及HepG2的增殖抑制和凋亡诱导作用。方法:以体外培养的人肝癌细胞HHCC和HepG2为研究对象,采用水浴加温法,观察单纯热疗,ADM化疗和热化疗对细胞增殖和凋亡的影响。MTT法确定阿霉素的工作浓度并检测细胞增殖的抑制作用,流式细胞术检测细胞凋亡。结果:热化疗组细胞的抑制率显著高于单纯热疗、单纯化疗组[HHCC细胞:(65.77±2.54)%vs(23.18±0.81)%、(38.35±2.23)%,P<0.05。HepG2细胞:(74.25±1.53)%vs(1 7.1 2±2.8 6)%、(30.35±5.90)%,P<0.05]。热化疗组细胞凋亡率显著高于单纯热疗组、单纯化疗组[HHCC细胞:(76.1±2.33)%vs(23.83±1.76)%、(45.57±2.81)%,P<0.05。HepG2细胞:(76.9±2.79)%vs(19.7±7.63)%、(37.43±1.88)%,P<0.05]。结论:加热能增强ADM对HHCC及HepG2的增殖抑制和诱导凋亡作用。

Proliferation and apoptosis induced by thermochemotherapy of adriamycin( ADM) in hepatocellular carcinoma cell line

Objective:To observe the effect of thermotherapy combined with adriamycin on cell proliferation and apoptosis of hepatocellular carcinoma cells.Methods: The working concentration of adriamycin was determined by MTT assay.Cells were subjected to thermotherapy(at 43℃) and chemotherapy with adriamycin alone or in conjunction,and the cell survival rates were determined at 24h after the treatment.The cell growth inhibition effect was evaluated by MTT assay,and the apoptosis rates of HepG2 and HHCC were determined by flow cytometric analyses.Results: The IC50 of adriamycin was defined as its working concentration.The thermotherapy at 43℃ for 60 min in combination with chemotherapy significantly inhibited the growth of both HepG2 and HHCC cells.The results of flow cytometry analyses showed that thermotherapy and adriamycin chemotherapy,used either alone or in combination,obviously increased the apoptosis rates of HHCC and HepG2 cells(P<0.05).Conclusion: Adriamycin combined with thermotherapy for 60min can increase the inhibition of proliferation and the apoptosis rates of HepG2 and HHCC cells.