辅酶Q10对大鼠视网膜光损伤的防护作用研究

目的观察辅酶Q10对实验性大鼠视网膜光损伤的防护作用及其机制。方法30只Sprague-Dawley(SD)大鼠随机分为正常对照组、阳性对照组、辅酶Q10组等3组，用（2000±120）lx绿色荧光灯对SD大鼠进行24 h持续照射，建立视网膜光损伤模型。于光照前24 h、30 min分别通过阳性对照组和辅酶Q10组大鼠尾静脉注射生理盐水和辅酶Q10。光照后第1天常规摘除眼球,光学和电子显微镜观察视网膜组织病理学改变；流式细胞术检测视网膜细胞凋亡率。结果组织病理学检查显示，辅酶Q10组外核层排列整齐，仅见少量凋亡细胞,内、外节轻度水肿，与阳性对照组比较，光损伤后的组织病理改变明显减轻；流式细胞术检测显示，正常对照组视网膜细胞凋亡率为（1.65±1.48）%，阳性对照组为（25.83±2.92）%，辅酶Q10组为（12.43±2.25）%。阳性对照组视网膜细胞凋亡率较正常大鼠视网膜明显升高(t=18.28，P＜0.01),辅酶Q10组的视细胞凋亡率较阳性对照组明显降低（t=9.07，P＜0.01）。结论辅酶Q10对光损伤引起的视网膜损害及视细胞凋亡有较好的防护作用。（中华眼底病杂志，2007，23：122-125）

The protective effect of coenzyme Q10 on retinal photic injury in rats

ObjectiveTo explore the protective mechanism and effect of coenzyme Q10 on the retinal photic injury in experimental rats.MethodsThirty Sprague-Dawley rats were divided randomly into 3 groups: normal control group, positive control group, and coenzyme Q10 group. The experimental model of photic injury in rats was established by being exposed to intense green fluorescent light with an illuminance level of (2000±120) Lux for 24 hours. The physiological saline and coenzyme Q10 were given through tail intravenous injection at 24 hours and 30 min before light exposure in positive control group and coenzyme Q10 group, respectively. Ophthalmectomy was performed 1 day after the illumination. Changes of retinal histopathology and ultrastructure were observed by light and electron microscope. The apoptosis rate of retinal cells was detected by flow cytometry.ResultsThe result of histopathological examination showed that in coenzyme Q10 group, the outer segments arranged trimly with only few cell apoptosis; the inner and outer segments slightly swelled, and compared with the positive group, the histopathological changes alleviated obviously. The result of flow cytometry revealed that the apoptosis rate of retinal cells was (1.65±1.48)% in normal control group, (25.83±2.92)% in positive control group, and (12.43±2.25)% in coenzyme Q10 group, respectively. The apoptosis rate of retinal cells was higher in positive control group than that in the normal control group (t=18.28, P<0.01), and lower in coenzyme Q10 group than that in the positive control group (t=9.07, P<0.01).ConclusionCoenzyme Q10 plays an important role in preventing the photic injury of retina and optic cell apoptosis.(Chin J Ocul Fundus Dis, 2007, 23: 122-125)