邻苯二甲酸二乙基已酯对未成年小鼠卵巢发育影响

目的观察邻苯二甲酸二乙基己酯(DEHP)对卵泡生长发育和性激素分泌影响,探讨DEHP对未成年雌性小鼠卵巢发育的毒性作用。方法将60只未成年(3周龄)清洁级ICR雌性小鼠随机分为4组,分别为对照(玉米油)组和低、中、高剂量 DEHP染毒组(100、400、1 600 mg/kg),每组15只,采用灌胃方式染毒,染毒容量为0.1 mL/kg,每天1次,每周5 d,连续6周;染毒结束后,检测小鼠血清促卵泡生成激素(FSH)、促黄体生成激素(LH)、雌激素、孕激素水平,取出小鼠卵巢组织连续切片观察卵巢各级卵泡数目构成比。结果 高剂量DEHP组血清中FSH水平为(0.72±0.04) mIU/mL 高于对照组的 (0.58±0.30) mIU/mL,差异有统计学意义(P<0.05);与对照组[(1.74±0.17) pg/mL]比较,低、中剂量DEHP组小鼠血清中雌激素水平[(1.62±0.18)、(1.69±0.16) pg/mL]均降低,高剂量组[(2.11±0.04) pg/mL]升高,差异均有统计学意义(P<0.05);各剂量染毒组LH和孕激素水平与对照组比较差异均无统计学意义。结论 DEHP暴露对未成年雌性小鼠的卵巢发育具有毒性作用。

Impact of DEHP on development of ovary in immature female mice

Objective To study the impacts of di-(2-ethylhexyl) phthalate(DEHP) on expressions of hormone including estradiol (E₂) and progesterone (P₄),and follicle development in ovary of immature mice.Methods Sixty ICR female mice were randomly divided into 4 groups:a negative control,three experimental groups treated with DEHP at doses of 100,400, and 1 600 mg/kg,though stomach gavage onceA day and 5 days a week for 6 consecutive weeks. After the treatment, the mice's ovaries were prepared to detect te developments of follicles.Sera of the m ice were sampled to measure the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), E₂ and P₄.Results FSH level (0.72±0.04 mIU/ml) in high dosage group was higher then that of the control group (0.58±0.30 mIU/ml) (P<0.05).E₂ levels in low and medium dose groups (1.62±0.18 and 1.69±0.16 pg/mL) were significantly lower,but significantly higher in high dose DEHP group than that of the control group (P<0.05 for all).Conclusion DEHP has obvious development toxicity to ovary of immature female mice.