用聚乙二醇沉淀法筛查高泌乳素血症的应用评价

目的探讨用聚乙二醇（PEG）沉淀法去除假性高泌乳素（PRL）血症的临床应用评价。方法测定232例高值PRL患者及52名正常体检者血清PRL,血清经PEG沉淀后再次测定其PRL,同时用缓冲液沉淀做对照,并对二者结果进行比较。结果高值PRL组中巨PRL检出率为25．4％,正常对照组中巨PRL的检出率为0．0％,2组差异有统计学意义（P〈0．01）。单体PRL组PEG处理前后血清PRL差异无统计学意义（P〉0．05）,而巨PRL组和可疑巨PRL组PEG处理前后PRL差异有统计学意义（P〈0．01）。3组经缓冲液处理前后PRL差异无统计学意义（P〉0．05）。结论高值PRL部分是假性升高,用PEG沉淀法筛查高值PRL对提高临床诊断的准确性有一定的价值,能减少对高PRL血症的误诊。

Evaluation of polyethylene glycol precipitation test as a screening method for hyperprolactinemia

Objective To eliminate false hyperprolactinemia by polyethylene glycol （PEG） precipitation test. Methods Prolactin （PRL） was detected in sera before and after precipitation by PEG from 232 patients with hyperpro- lactinemia and 52 healthy controls. The results of PRL were compared with that of a buffer control group at the same time. Results The positive rate of macroprolactinemia in hypeiprolactin group and healthy controls was 25.4% and 0.0% respectively. The differences between the two groups were statistically significant（P 〈 0.01 ）. Before and after treatment with PEG, the level of PRL in monoprolactin group was not statistically significant （P 〉 O. 05）, while that in the macroprolactin group and the suspicious macroprolactin group were statistically significant （P 〈 0. O1 ）. Before and after treatment with buffer, the serum levels of PRL in three groups were not statistically significant （ P 〉 0.05 ） . Con- clusions There is false hyperprolactinemia. PEG precipitation test is valuable to improve the accuracy of hyperprolactin determination and reduce the misdiagnosis of hyperprolactinemia.