低氧性肺动脉高压大鼠肺组织中介素的分布

目的观察低氧性肺动脉高压大鼠血浆中介素含量及肺组织中介素的分布。方法成年雄性Wistar大鼠被随机分为对照组，低氧1w组、2w组、3w组。对照组置于舱外常氧环境中饲养；其他3组动物置于低压舱内，模拟海拔5000m高度缺氧，每天连续7h。各组大鼠颈总动脉取血，应用放射免疫法检测血浆中介素含量，用免疫组织化学法检测中介素在肺内的分布。结果对照组，低氧1w组、2w组、3w组血浆中介素含量依次为（138．6±5．3）、（154．6±20．2）、（158．8±21．3）、（166．3±12．2）pg／ml，低氧各组大鼠血浆中介素含量增高。低氧3w时升高最明显，明显高于对照组（P〈0．05）。中介素在大鼠肺组织多种细胞中广泛分布。结论中介素在肺动脉高压的病理生理过程中发挥显著作用。

Distribution of intermedin in lung tissues of rats with hypoxic pulmonary arterial hypertension

Objective To observe the contents of intermedin in the plasma of rats with hypoxic pulmonary arterial hypertension and the distribution of intermedin in their lungs tissues.Methods Adult male Wistar rats were randomly divided into control group,hypoxia for 1 w group,hypoxia for 2 w group,and hypoxia for 3 w group.Rats in the control group were bred in the outboard normoxia environment,and ones in the other three groups were bred in the low pressure chamber which simulated the highly hypoxic environment of 5000 m above the sea level for continuous 7 h everyday.Blood samples were obtained from common carotid artery.Radio-immunity method was used to detect the plasma intermedin,and immunohistochemical method was used to detect the distribution of intermedin in lungs.Results The contents of plasma intermedin in the control group,hypoxia for 1 w group,hypoxia for 2 w group,and hypoxia for 3 w group were respectively(138.6±5.3),(154.6±20.2),(158.8±21.3),and(166.3±12.2)pg/ml.The contents in the hypoxic groups all increased,and the increased content in the hypoxia for 3 w group was the most obvious,which was significantly higher than that in the control group(P<0.05).The intermedin existed in many kinds of cells of rats’ lung tissues.Conclusion Intermedin plays a significant role in the pathological and physiological development of hypoxic pulmonary arterial hypertension.