休克大鼠小肠杯状细胞参与肠粘膜重建的形态学观察

目的观察大鼠失血性休克复苏后小肠杯状细胞在参与肠粘膜重建过程中的形态学变化。方法SD大鼠35只,每只重250～300g,随机分为实验和对照两组,实验组再分为休克复苏后1h、3h、6h、12h组;各组留取回肠粘膜,观察休克复苏后早期不同阶段肠粘膜的形态改变、杯状细胞数量变化。结果休克后1h可见肠粘膜明显损伤,3h起肠粘膜呈现损伤后重建现象,可见杯状细胞聚集于损伤的肠粘膜表面,12h后大部分粘膜细胞被呈过分泌状态的杯状细胞覆盖,肠粘膜表面细胞连续性得到恢复;肠粘膜杯状细胞数量在肠粘膜重建过程内呈下降趋势。结论肠粘膜损伤后早期的重建过程中杯状细胞可能起关键作用。

Observation of morphology on small intestinal goblet cells joining reconstruction of intestinal shock rats

Objective To investigate the morphological changes of the goblet cells in contributing to the intestinal mucosal reconstrution during the resuscitation process after hemorrhagic shock in rats. Methods 35 Sprague-Dawley rats with body weight of 250-300g were randomly divided into 2 groups: Control group (sham shock) and Experimental group. Experimental group was further randomly divided into 4 groups according to different time after hemorrhagic shock resuscitation, including 1st hour group, 3rd hour group, 6th hour group and 12th hour group. After experimental rat model of hemorrhagic shock was set up, the specimens from ileum tissue were taken respectively at 1, 3, 6 and 12h after shock resuscitation. The morphological observation of the intestinal mucosa and the number of goblet cells were determined. Results After rat hemorrhagic shock, the mucosal epithelial injury was obvious in the small intestine and was becoming more serious at the 1h and the 3h. The tissue reconstrution was also found after 3h. Most of the injured mucosal reconstrution were established in 6h to 12h. Following tissue reconstrution, the denuded mucosal surface was covered intensively by goblet cells. The number of goblet cells on intestinal mucosa was reduced significantly from the 1h to the 12h group. Conclusion It is possible that goblet cell plays a key role on the reconstrution of intestinal mucosa though their amount was decreased.