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Nuclear body formation and PML body remodeling by the human cytomegalovirus protein UL35
Authors:Salsman Jayme  Wang Xueqi  Frappier Lori
Affiliation:
  • Department of Molecular Genetics, 1 Kings College Circle, University of Toronto, Toronto, Ontario, Canada M5S 1A8
  • Abstract:The human cytomegalovirus (HCMV) UL35 gene encodes two proteins, UL35 and UL35a. Expression of UL35 in transfected cells results in the formation of UL35 nuclear bodies that associate with promyelocytic leukemia (PML) protein. PML forms the basis for PML nuclear bodies that are important for suppressing viral lytic gene expression. Given the important relationship between PML and viral infection, we have further investigated the association of UL35 with PML bodies. We demonstrate that UL35 bodies form independently of PML and subsequently recruit PML, Sp100 and Daxx. In contrast, UL35a did not form bodies; however, it could bind UL35 and inhibit the formation of UL35 bodies. The HCMV tegument protein pp71 promoted the formation of UL35 bodies and the cytoplasmic localization of UL35a. Similarly, UL35a shifted pp71 to the cytoplasm. These results indicate that the interplay between UL35, UL35a and pp71 affects their subcellular localization and likely their functions throughout infection.
    Keywords:CMV   Cytomegalovirus   PML   Promyelocytic leukemia   pp71   UL35   UL35a   Nuclear bodies
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