中药益坤宁对围绝经期大鼠卵巢血管内皮生长因子表达的影响

目的:观察中药益坤宁对围绝经期大鼠卵巢血管内皮生长因子(Vascular endothelial growth factor,VEGF)表达的影响。方法:采用自然老化法建立围绝经期大鼠模型。选择处于围绝经期的雌性Wistar大鼠(12~14月龄)30只,随机分为老年对照组、西药对照组和中药实验组,分别以生理盐水、利维爱和益坤宁灌胃4周;另取5月龄大鼠10只作为青年对照组,生理盐水灌胃4周。通过免疫组化观察大鼠卵巢VEGF蛋白质的表达确切部位;Western Blot定量检测大鼠卵巢VEGF蛋白质的表达;RT-PCR检测各组大鼠卵巢VEGF mRNA的表达。结果:VEGF蛋白主要表达于卵巢的黄体细胞中,老年对照组大鼠卵巢的VEGF表达明显高于青年组,中药实验组大鼠卵巢VEGF表达明显下降;青年对照组、中药实验组和西药对照组VEGF164和VEGF120的mRNA表达量均低于老年对照组(P<0.05),此结果与VEGF蛋白表达的结果相一致。结论:益坤宁能够下调围绝经期大鼠卵巢VEGF mRNA及蛋白的表达,这可能是其治疗围绝经期综合征的机制之一。

Effects of Chinese Medicine Yikunning on the Expression of Vascular Endothelial Growth Factor in Rat Ovaries During Perimenopause Period

Objective:To investigate the effect of traditional chinese medicine Yikunning on the expression of vascular endothelial growth factor (VEGF) in rat ovaries during perimenopause period. Methods:The animal model of perimenopause rats was established by unforced aging.30 female Wistar rats of 12 months old were randomly divided into three groups:old controls,western medicine controls,chinese medicine experimental group.10 female rats of 5 months old served as young controls.The animals were given with corresponding drugs for 4 weeks. The site-specific expression of VEGF in ovaries of rats was detected by immunohistochemistry; the quantitative expression of VEGF in ovaries of rats was detected by Western Blot;the quantitative expression of VEGF mRNA in ovaries of rats were detected by RT-PCR. Results:Compared with young controls,the expressions of VEGF mRNA in ovaries of old controls increased significantly,while the expressions of VEGF in ovaries of chinese medicine experimental group decreased significantly.The expression of VEGF164 mRNA and VEGF120 mRNA of the young controls,chinese medicine experimental group and western medicine controls were lower than the expression of VEGF mRNA of old controls(P<0.05),which were consistent with the result of the expression of VEGF. Conclusions: Yikunning could down-regulate the expression of VEGF mRNA and protein in rat ovaries during perimenopause period,which may be one of the mechanisms of Yikunning to treat perimenopausal syndrome.