自噬基因Beclin 1在宫颈鳞癌中的蛋白表达及其临床意义

目的探讨自噬基因Beclin 1在宫颈鳞癌中的蛋白表达以及与肿瘤发生发展的关系。方法采用免疫组化SP法检测81例宫颈鳞癌、20例宫颈上皮内瘤样病变（CIN，Ⅱ～Ⅲ级）和20例正常宫颈组织中自噬基因Beclin 1的蛋白表达。结果宫颈鳞癌组织中Beclin 1阴性、弱阳性、强阳性表达率依次是43．2％（35／81）、34．6％（28／81）、22．2％（18／81），与正常宫颈和宫颈上皮内瘤样病变组相比，Beclin 1在宫颈鳞癌中的表达显著下调（P=0．011）。宫颈鳞癌中Beclin 1的表达在不同FIGO分期、年龄、宫颈肌层浸润深度、宫颈肿瘤大小、大体类型间的差异无统计学意义。但在有无盆腔淋巴结转移、不同肿瘤分化程度间差异有统计学意义（P〈0．05）。结论自噬基因Beckn 1在宫颈鳞癌中蛋白表达下调，并与宫颈鳞癌盆腔淋巴结转移和肿瘤分化程度有关。

Expression and Clinical Significance of Autophagy Gene Beclin 1 in Cervical Squamous Cell Carcinoma

OBJECTIVE: Autophagy gene Beclin 1 plays an important role in several types of human cancers. So we, in this study, employed the immunohistochemical manner to detect the Beclin 1 gene expression and explore its clinical significance in cervical aquamous cell carcinoma. METHODS: SP immunohistochemistry technique was used to detect the expression of Beclin 1 gene in specimens of 81 cervical squamous cell carcinoma, 20 cervical intraepithelial neoplasm (CIN, II - III), and 20 normal cervix. Correlations between the expressions of Beclin 1 gene and the clinicopathologic factors of cervical squamous cell carcinoma were statistically analyzed. RESULTS: The rates of negative, weak, strong expression of Beclin 1 in cervical squamous cell carcinoma were 43.2% (35/81), 34.6% (28/81) or 22.2% (18/81) respectively, and the significantly lower than those in normal cervix and CIN II - III (P = 0.011). The Beclin 1 expressions did not associate with FIGO stage, age, depth of cervical infiltration, tumor size, and gross type of cervical lesion (P < 0.05), but were related to pelvic lymph node metastases and histological tumor grade (P < 0.05). CONCLUSION:s Expression of autophagy gene Beclin 1 decreases in cervical aquamous cell carcinoma, and is closely related to pelvic lymph node metastases and histological tumor grade.