高脂高糖饮食诱导的2型糖尿病大鼠血清瘦素水平变化的研究

目的 研究分析2型糖尿病大鼠血清瘦素水平的变化及其与血糖、胰岛素之间的关系。方法 用自动生化分析仪分别测定实验组（由高脂高糖饲料与链脲佐菌素注射液诱导而成）和对照组（用常规饲料喂养）大鼠的血糖等各项生化指标，用放射免疫分析法分别测定实验组和对照组大鼠的瘦素、胰岛素等指标并进行分析。结果 实验组大鼠的胰岛素水平与对照组无显著差异（P=0．9940），瘦素水平则显著低于对照组（P=0．0042）：而血糖、总胆固醇则实验组明显高于对照组（P=0．015、0．0109）。结论 大鼠在2型糖尿病状态下，高血糖抑制胰岛素刺激脂肪组织分泌瘦素的效应而使瘦素水平降低。

The serum leptin levels in induced type 2 diabetic rats

Objective To investigate the change of serum leptin levels and its relation with serum glucose and insulin levels in induced type 2 diabetic rats. Methods The serum levels of leptin and insulin were detected by radioimmunoassay. The levels of glucose, CHO and TG were detected by biochemical autoanalyzer. Results There was no significant difference of the serum insulin levels between type 2 diabetic rats and in controls(P=0.994). But the serum leptin levels in rats with type 2 diabetes was lower than those in controls(P=0.0042), and the serum glucose, CHO levels in rats with type 2 diabetes was higher than those in control rats(P=0.015,P=0.0109). Conclusion Hyperglycemia can inhibit insulin to stimulate leptin secretion in type 2 diabetic rats.