桑白皮中桑辛素的含量测定

目的：对不同产地,不同品种及规格的桑白皮中的桑辛素进行含量测定。方法：采用高效液相色谱法分析,Alltima C₁₈色谱柱(4.6 mm×250 mm,5 μm)；流动相乙腈-水(58∶42),检测波长270 nm。结果：未经除去黄棕色外皮的桑白皮样品与除去外部黄棕色外皮的样品中桑辛素含量差异明显,样品中的桑辛素主要存在于桑白皮的黄棕色外皮中。桑白皮桑辛素含量与样品厚度与有关。结论：本方法简便,稳定,为桑白皮质量控制提供参考依据。

Determination of morusin in Cortex Mori

OBJECTIVE: To determine the contents of morusin in Sangbaipi (Cortex Mori) of different commercial grades and species and obtained from different regions. METHOD: Contents of morusin were determined by HPLC on an Alltima C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water (58:42) as mobile phase. UV detection was conducted at 270 nm. RESULT: The amounts of morusin were found to vary among samples of Cortex Mori bearing a yellow-brown outer layer and those without the yellow-brown cork layer. The morusin contents were also related to the thickness of Cortex Mori. CONCLUSION: The method was simple, reproducible and reliable. It can be applied to the quality assessment of Cortex Mori.