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| 神经干细胞对谷氨酸兴奋毒性损伤神经元的保护作用 | |
| 引用本文： | 李进,陈江海,洪光祥,王发斌,康皓. 神经干细胞对谷氨酸兴奋毒性损伤神经元的保护作用[J]. 华中科技大学学报(医学版), 2007, 36(3): 304-306 |
| 作者姓名： | 李进 陈江海 洪光祥 王发斌 康皓 |
| 作者单位： | 华中科技大学同济医学院附属协和医院手外科,武汉,430022 |
| 摘 要： | 目的 研究未分化状态的神经干细胞对损伤脊髓神经元的直接保护作用。方法 建立体外大鼠脊髓神经元谷氨酸损伤细胞模型，在实验组中加入神经干细胞，对照组中加入培养液。通过MTT比色试验、彗星试验检测神经干细胞对神经元的保护作用。结果 MTT比色试验表明实验组神经元活力明显高于对照组，彗星试验表明实验组拖尾率和彗尾长度均低于对照组。结论 神经干细胞能有效保护谷氨酸损伤的脊髓神经元，其作用可能是通过自分泌、旁分泌因子产生的。 |
| 关 键 词： | 神经干细胞 脊髓神经元 神经元损伤 神经元保护 |
| 修稿时间： | 2006-02-30 |
Protective Effect of Neural Stem Cells on Spinal Cord Neurons in Glutamic Acid Damaged Models |
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| Li Jin, Chen Jianghai, Hong Guangxiang et al. Protective Effect of Neural Stem Cells on Spinal Cord Neurons in Glutamic Acid Damaged Models[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2007, 36(3): 304-306 | |
| Authors: | Li Jin Chen Jianghai Hong Guangxiang et al |
| Affiliation: | Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 |
| Abstract: | Objective To explore the protective effect of neural stem cells on spinal cord neurons. Methods Rat neural stem cells and rat spinal cord neurons were cultured, and glutamic acid neuron damaged models were created. Neural stem cells were added into experimental groups, and single culture medium was added into control groups. By using MTT assay and Com- et assay, the effect of neural stem cells on damaged spinal cord neurons was studied. Results After addition of neural stem cells, survival rate and DNA damaged levels in experimental groups were improved obviously. Conclusion Neural stem cells can protect damaged, spinal cord neuron by glutamate via autocrine or paracrine factors. |
| Keywords: | neural stem cells spinal cord neurons neuron injury neuron protection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |