硫辛酸对高脂诱导肥胖大鼠氧化应激影响的实验研究

目的探讨硫辛酸（LA）对高脂诱导肥胖大鼠氧化应激水平的影响。方法 29只5~6周龄Wistar雄性大鼠,按体重随机分为2组：基础组（n=8）和高脂组（n=21）。高脂组大鼠饲养6周末尾尖采血,测定空腹血糖,按血糖水平随机分为2组：高脂组,高脂＋硫辛酸干预组（硫辛酸组）,分别用生理盐水和LA进行腹腔注射,4周后测定大鼠体重、内脏脂肪含量以及血清丙二醛（MDA）、超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）和全血谷胱甘肽过氧化物酶（GSH-Px）。结果与基础组比较,高脂组大鼠的血清MDA水平和SOD活性明显增高,T-AOC下降,差异均有统计学意义（P〈0.05）;与高脂组比较,LA组大鼠体重和内脏脂肪含量明显降低,血清T-AOC明显升高,差异有统计学意义（P〈0.05）;三组大鼠GSH-Px差异无统计学意义（P〉0.05）。结论 LA能显著降低高脂诱导肥胖大鼠的体重和内脏脂肪含量,提高肥胖大鼠的抗氧化能力。

Experimental Study on Effect of Lipoic Acid on Oxidative Stress Levels in Obesity Rats Induced by High-fat Diet

Objective To explore the effect of lipoic acid(LA) on levels of oxidative stress in high-fat-diet-induced obesity in rats. Methods According to body weight,29 5～6-week-old male Wistar rats were randomly divided into basic diet group(n=8) and high-fat diet group(n=21).After 6 weeks,the rats in high-fat diet group were determined the fasting blood sugar level,and then they were divided into high-fat group and LA group according to the level of blood sugar.The rats in the two groups were intraperitoneally injected with normal saline or LA,respectively.After 4 weeks,the rats’ body weight,visceral adipose mass and serum malondialdehyde(MDA),superoxide dismutase(SOD),total antioxidant capacity(T-AOC) and whole blood glutathione peroxidase (GSH-Px) were determined. Results Compared with basic diet group,the serum MDA level and SOD activity in high-fat group were increased significantly,but serum T-AOC level decreased significantly,and the difference was statistically significant(P<0.05).Compared with high-fat group,the rats’ body weight and visceral adipose composition in LA group were decreased significantly,but serum T-AOC level increased significantly,and the difference was statistically significant(P<0.05).There was no statistically significant difference in GSH-Px among the rats in three groups(P>0.05). Conclusions LA can significantly reduce body weight and visceral adipose mass of fat-induced-obesity rats and increase their anti-oxidantive ability.