亚慢性砷暴露对大鼠肾脏功能和形态的影响

目的观察亚慢性亚砷酸钠暴露对大鼠肾脏结构和功能的影响。方法 27只雄性SD大鼠,随机分对照组,砷暴露低剂量组和砷暴露高剂量组,分别自由饮用去离子水和亚砷酸钠水溶液,时间为3个月。观察肾脏组织,采用全自动生化分析仪检测肾功,苏木精-伊红染色（HE染色）检测组织病理学改变,电镜观察超微结构改变。结果①生化分析发现尿素氮和葡萄糖水平随染毒剂量增加而显著增加。②HE染色镜检发现砷暴露组大鼠肾小管发生空泡样变、嗜酸性变,损伤效应具有剂量依赖性,且发现有炎性细胞的浸润。③电镜检查发现砷暴露组大鼠肾小球有轻度的系膜增生。结论亚慢性饮水染砷可对肾脏功能产生显著影响,导致肾小球系膜轻度增生和肾小球空泡样变。

Effect of Chronic Arsenic Administration on Renal Function and Morphology in Rats

Objective To observe the effect of chronic sodium arsenite exposure on the renal function and morphological structure in rats.. Methods Twenty-seven male SD rats were randomly divided into control group,low-dose sodium arsenite exposure group,and high-dose sodium arsenite exposure group.Sodium arsenite was administrated by adding to drinking water for 3 months.The renal function was determined by an automatic biochemistry analyzer.The morphological changes of kidney were observed by a microscope following hematoxylin and eosin staining,and the ultra-structural changes were observed by an electron microscope. Results Altered kidney functions were found in the sodium arsenite-exposed rats by biochemical analysis.Sodium arsenite exposure also resulted in vacuolar degeneration and eosinophilic change in renal tubular,and invasion of inflammatory cells in the high-dose group.Furthermore,mild mesangial proliferation in renal glomerulus was observed in sodium arsenite-exposed groups. Conclusions Chronic sodium arsenite administration through drinking water had a harmful effect on renal function,and it could induce mild proliferation in renal glomerulus mesangial cell and renal tubular vacuolar degeneration in rats.