首页 | 本学科首页   官方微博 | 高级检索  
     


Chemically induced Salmonella enteritidis ghosts as a novel vaccine candidate against virulent challenge in a rat model
Authors:Nagarajan Vinod  Sung Oh  Seongdae Kim  Chang Won Choi  Sei Chang Kim  Cheong- Hwan Jung
Affiliation:1. Department of Biology & Medicinal Science, Pai Chai University, Daejeon 302-735, Republic of Korea;2. Technology Research Institute, KBNP, Inc., Yesan, Chungnam 340-861, Republic of Korea
Abstract:
Salmonella enteritidis ghosts (SEGs), non-living empty bacterial cell envelopes were generated by using the minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH) and investigated as a vaccine candidate in rats. To determine the immunogenicity and protective efficacy of SEG vaccine, rats were divided into four groups: group A (non-vaccinated control), group B (orally vaccinated), group C (intramuscularly vaccinated) and group D (intramuscularly vaccinated with complete Freund's adjuvant). Vaccination of rats with SEGs induced significant immune responses before and after virulent challenge. Rats vaccinated with SEGs showed significant increases in serum IgG antibodies after challenging with virulent S. enteritidis on week 8 and week 10 (P < 0.01). During the vaccination period, groups B, C and D showed significantly higher serum bactericidal activity (SBA) compared to group A (P < 0.01). Most importantly, bacterial loads in vaccinated groups were significantly lower than in the non-vaccinated group (P < 0.01). In conclusion, these results show that the chemically induced SEGs as a vaccine candidate against virulent challenge.
Keywords:Salmonella enteritidis   Bacterial ghosts (BGs)   Vaccine candidate   Protective immunity
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号