Snail在17β雌二醇所引起的卵巢透明细胞癌ES-2细胞系侵袭力增加中的作用

 目的 转录因子Snail可诱发上皮细胞-间充质细胞转化（epithelial-mesenchymal transitions,EMT），在肿瘤的黏附、侵袭、转移中起重要作用。本研究通过RNA干扰抑制Snail的表达，观察Snail表达抑制后是否影响17β雌二醇对卵巢透明细胞癌细胞系ES-2细胞趋向运动力、侵袭力及MMP-2表达的调控。方法 构建针对Snail的dsRNA表达载体pRNAT-U6.1/Neo-Snail，瞬时转染抑制Snail表达。10-8mol/L 17β雌二醇及DMSO分别作用于阴性对照组及RNAi组细胞，用改良的Boyden小室检测细胞侵袭力、运动力的变化，RT-PCR检测MMP-2 mRNA表达的改变情况,明胶酶谱检测基质金属蛋白酶活性。 结果 用针对Snail的RNAi阻断Snail表达后，可使ES-2细胞胞质突起变短，细胞的趋向运动力、侵袭力及MMP-2表达降低；针对Snail的RNAi可部分阻断17β雌二醇对ES-2细胞趋向运动力、侵袭力的调节，并可阻断17β雌二醇对细胞MMP-2表达的调节作用。 结论 Snail在雌激素引起的卵巢透明细胞癌肿瘤细胞侵袭能力增强的过程中起重要作用，可作为卵巢透明细胞癌治疗的重要靶点。

Effects of different doses of oxycodone combined with propofol and remifentanil on the patients undergoing hysteroscopy surgery

Objective To observe the clinical effects of different doses of oxycodone combined with propofol and remifentanil on the patients undergoing hysteroscopy surgery. Methods Three hundred patients,aged between 18 to 60,with body mass index (BMI) of 18-24 kg/m²,of ASA physical status I or Ⅱ were enrolled in the study and randomly divided into 5 groups by a random number table (n=60).Remifentanil of 1 μg/kg (group R),sufentanil of 0.5 μg/kg (group S) and oxycodone of 0.05,0.10,0.15 mg/kg combined with propofol and remifentanil (group O_1-3) was infused by vein,respectively.All patients in each group were induced under general anesthesia by vein injection of oxycodone,remifentanil or sufentanil followed by 2 mg/kg of propofol infusion.Succinylcholine of 1 mg/kg was administered for muscle relaxation prior to intubation.All patients were mechanically ventilated after intubation with the laryngeal mask.Anesthesia was maintained with infusion at the rate of 6-12 mg·kg^-1·h^-1 for propofol and remifentanil 0.25 μg·kg^-1·min^-1.Then we observed and recorded the occurrence of cough,hypertension,bradycardia,tachycardia,body movement and cardiovascular events,respectively.Flurbiprofen 50 mg was administrated when the VAS score was 4 grade and above,and the incidence of nausea and vomiting were recorded. Results Compared with group R and S,the incidence of hypotension,bradycardia,nausea and vomiting,and the usage of flurbiprofen significantly decreased in group O_1-3 (P<0.05).Compared with group R,the incidence of hypertension and tachycardia significantly decreased in group O₁,and postanaesthetic recovery time significantly decreased in the other 4 groups (P<0.05).Compared with group S,the incidence of cough significantly decreased in group O_1-3 (P<0.05),the incidence of hypertension and tachycardia significantly increased in group O₁ (P<0.05).Compared with group O₁,the incidence of hypertension and tachycardia and the usage of flurbiprofen significantly decreased in group O₂ and O₃ (P<0.05),the incidence of bradycardia significantly increased in group O₃,and the incidence of body movement and propofol dosage significantly decreased in the other 4 groups. Conclusions Oxycodone of 0.10-0.15 mg/kg combined with propofol and remifentanil can be safely and effectively used in hysteroscopy surgery as an alternative anesthesia method,which characterized by rapid recovery from anesthesia and ideal postoperative analgesia with mild adverse effects.