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| 肢体缺血后处理通过激活MAPK途径保护大鼠海马区缺血性神经元 | |
| 引用本文： | 姜红升,涂悦,程世翔,王毅,令狐海瑞,张赛. 肢体缺血后处理通过激活MAPK途径保护大鼠海马区缺血性神经元[J]. 解剖科学进展, 2011, 17(1): 4-7 |
| 作者姓名： | 姜红升 涂悦 程世翔 王毅 令狐海瑞 张赛 |
| 作者单位： | 1. 天津医科大学研究生院,天津,300070;武警医学院附属医院脑创伤与神经疾病研究所,天津,300162 2. 武警医学院附属医院脑创伤与神经疾病研究所,天津,300162 3. 天津医科大学研究生院,天津,300070 |
| 基金项目： | 武警医学院院部重点项目 |
| 摘 要： | 目的探讨肢体缺血后处理对缺血性神经元损伤的作用,观察大鼠海马CA1区ERK1/2的磷酸化及其蛋白表达的变化情况。方法健康成年雄性SD大鼠,随机分为假手术组(sham)、全脑缺血再灌组(四动脉闭塞全脑缺血模型ischemia/reperfusion,I/R)、肢体缺血后处理组(limb ischemia postconditioning,Lpost)、肢体缺血后处理+ERK1/2抑制剂组(Lpost+PD98059)。光镜下焦油紫染色法观察各组大鼠海马CA1区神经元存活情况;Western blot法检测海马CA1区ERK1、2磷酸化及蛋白表达情况。结果 Western blot结果显示脑缺血再灌注各时间点Lpost组p-ERK1/2水平均较I/R组高,且于再灌注后10min、1d出现两个高峰,给予ERK1/2上游激酶抑制剂PD98059后能够抑制再灌注后1dp-ERK1/2水平的升高;而各时间点各组ERK1/2水平之间无明显差异。焦油紫染色结果显示再灌注1d后,I/R组与sham组相比,海马CA1区存活神经元数量明显减少;Lpost组较I/R组海马CA1区存活神经元数量明显增加;而给予ERK1/2上游激酶抑制剂PD98059后(Lpost+PD98059)神经元生存数量较Lpost组大幅下降。结论肢体缺血后处理具有抗大鼠海马CA1区缺血性神经元损伤的作用,激活ERK1/2通路可能是其发挥保护作用机制之一。 |
| 关 键 词： | 缺血再灌注损伤 肢体缺血后处理 ERK1/2 SD大鼠 |
The protective effect of limb ischemia postconditioning on ischemic neurons of rat hippocampus by activating MAPK pathway |
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| JIANG Hong-sheng,TU Yue,CHENG Shi-xiang,WANG Yi,LINCHU Hai-rui,ZHANG Sai. The protective effect of limb ischemia postconditioning on ischemic neurons of rat hippocampus by activating MAPK pathway[J]. Progress of Anatomical Sciences, 2011, 17(1): 4-7 | |
| Authors: | JIANG Hong-sheng TU Yue CHENG Shi-xiang WANG Yi LINCHU Hai-rui ZHANG Sai |
| Affiliation: | JIANG Hong-sheng1,2,TU Yue2,CHENG Shi-xiang2,WANG Yi2,LINGHU Hai-rui1,ZHANG Sai2* ( 1. Graduate School of Tianjin Medical University,Tianjin 300070,2. Department of Neurosurgery,Affiliated Hospital of Medical College of Chinese People's Armed Police Force,Tianjin 300162 China) |
| Abstract: | Objective To elucidate the protective effect of limb ischemia postconditioning on ischemic neurons of rat hippocampus and the possible mechanism related to ERK1/2 phosphorylation and protein expression in hippocampal CA1 region of the rats. Methods Adult male SpragueDawley rats were randomly divided into four groups:sham, ischemia/reperfusion(global cerebral ischemia by four-vessels occlusion,I/R), limb ischaemia postconditioning(Lpost), and Lpost+PD98059 groups. Cresyl violet staining was used to observe s... |
| Keywords: | ERK1/2 |
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