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5-氟尿嘧啶诱导人肺腺癌A549细胞自噬
引用本文:潘效红,王娟,张瑾锦,闫苗苗,于敏. 5-氟尿嘧啶诱导人肺腺癌A549细胞自噬[J]. 解剖学报, 2011, 42(3): 374-377. DOI: 10.3969/j.issn.0529-1356.2011.03.016
作者姓名:潘效红  王娟  张瑾锦  闫苗苗  于敏
作者单位:滨州医学院药学院细胞工程教研室, 山东 烟台 264003
基金项目:滨州医学院科技计划资助项目,山东省高校科技计划资助项目
摘    要:
目的 探讨5-氟尿嘧啶(5-FU)是否诱导人肺腺癌A549细胞发生自噬.方法 吖啶橙(AO)荧光染色和透射电镜观察自噬泡的变化;细胞免疫化学法测定LC3-Ⅱ的表达;流式细胞术及吖啶橙染色结合激光扫描共焦显微镜检测细胞凋亡;DNA电泳检测凋亡梯形条带的产生.结果 吖啶橙荧光染色和透射电镜观察结果显示,5-氟尿嘧啶处理细胞...

关 键 词:5-氟尿嘧啶  肺腺癌  自噬  免疫细胞化学  流式细胞术  
收稿时间:2010-11-01

5-fluorouracil induced autophagy in human lung adenocarcinoma A549 cells
PAN Xiao-hong,WANG Juan,ZHANG Jin-jin,YAN Miao-miao,YU Min. 5-fluorouracil induced autophagy in human lung adenocarcinoma A549 cells[J]. Acta Anatomica Sinica, 2011, 42(3): 374-377. DOI: 10.3969/j.issn.0529-1356.2011.03.016
Authors:PAN Xiao-hong  WANG Juan  ZHANG Jin-jin  YAN Miao-miao  YU Min
Affiliation:Department of Cell Engineering, School of Pharmaceutical Sciences, Binzhou Medical University, Shandong Yantai 264003, China
Abstract:
Objective To study if 5-fluorouracil (5-FU) can induce human lung adenocarcinoma A549 cells’ autophagy in order to provide theoretical basis for the tolerance of the chemotherapeutics. Methods Autophagic vacuole’s change was observed with acridine orange (AO) staining under fluorescent microscope and transmission election microscope(TEM); LC3-Ⅱ expression was tested with cellular immunochemistry; Apoptosis was detected by flow cytometry and AO staining with laser scanning confocal microscopy detection; Production of apoptosis’ ladder shaped stripe was tested with DNA cataphoresis. Results After being treated with 5-fluorouracil, the cells cytoplasm appeared to have apparent autophagic vacuoles when observed with AO staining and TEM observations; The cells showed that LC3-Ⅱexpression was apparently increased and 3-MA significantly inhibited the increase of LC3-Ⅱ when observed with cellular immunochemistry; The cell apoptosis did not occur when tested by flow cytometry, AO staining and DNA ladder. Conclusion Low concentration(5μmol/L) of 5-fluorouracil can
Keywords:5-fluorouracil  Lung adenocarcinoma  Autophagy  Immunocytochemistry  Flow cytometry  Human
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