S100A7表达下调对胃癌SGC-7901细胞增殖和迁移能力的影响

目的 研究S100A7表达下调对胃癌SGC-7901细胞增殖和迁移的影响,并探讨其可能的分子机制.方法 将胃癌SGC-7901细胞分为3组:未转染组、对照siRNA组和S100A7 siRNA组,后两组分别转染对照siRNA和S100A7 siRNA.利用实时荧光定量RT-PCR和蛋白质印迹分析检测各组胃癌SGC-7901细胞中S100A7 mRNA和蛋白的表达;采用CCK-8和Boyden小室分别检测各组细胞增殖和细胞迁移能力的变化.最后采用蛋白质印迹分析法检测3组胃癌细胞中cyclin D1、Cdk2和MMP-2蛋白的表达.结果 S100A7 siRNA能下调胃癌SGC-7901细胞中S100A7 mRNA和蛋白的表达.S100A7 mRNA和蛋白表达下调抑制胃癌SGC-7901细胞的增殖和迁移,并降低cyclin D1、Cdk2和MMP-2蛋白的表达.结论 S100A7表达下调介导的胃癌细胞增殖抑制和迁移降低可能与cyclin D1、Cdk2和MMP-2表达的下调密切相关.

Effect of down-regulated S100A7 expression on proliferation and migration of gastric carcinoma cell line SGC-7901

ObjectiveTo investigate the effect of down-regulated S100A7 expression on proliferation and migration of gastric carcinoma SGC-7901 cells, and to explore the possible molecular mechanisms. MethodsThe experiment was divided into three groups, including untransfected group, control siRNA group and S100A7 siRNA group. S100A7 siRNA and control siRNA were transfected into gastric carcinoma SGC-7901 cells in S100A7 siRNA group and control siRNA group, respectively. Expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells were examined by Real-time PCR and Western blotting analysis, respectively. CCK-8 kit and Boyden chamber were used to observe the proliferation and migration of SGC-7901 cells in the three groups. The expressions of cyclin D1, Cdk2 and MMP-2 proteins were also examined by Western blotting analysis in the three groups. ResultsS100A7 siRNA effectively down-regulated the expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells. Down-regulated S100A7 expression greatly inhibited the proliferation and migration of gastric carcinoma SGC-7901 cells, and also down-regulated expression of cyclin D1, Cdk2 and MMP-2 proteins. ConclusionDown-regulated S100A7 expression-mediated inhibition of gastric carcinoma cell proliferation and migration may be closely associated with the decreased expressions of cyclin D1, Cdk2 and MMP-2 proteins.