Inhibition of cytotoxicity for screening a monoclonal antibody to HLA antigen |
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| Authors: | Shingo Hiroishi Shigeyuki Matsuyama Toshiaki Kaneko Yukiyo Nishimura Junichiro Arita |
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| Affiliation: | Division of Clinical Diagnostics, Shiraimatsu Shinyaku Co., Ltd., Shiga, Japan |
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| Abstract: | ![]()
Cytotoxicity inhibition assay was established for the screening of a monoclonal antibody to HLA antigen. The assay involved the inhibition of typing cells with hybridoma culture supernatant and with F(ab')2 fragment of sheep anti-mouse IgG. Using the assay and the conventional microcytotoxicity test, an anti-HLA-A2 monoclonal antibody was screened. |
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