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| 姜黄素对肝纤维化模型大鼠肝纤维化组织细胞因子的影响 | |
| 引用本文： | 张小斌,曾令兰,陈莎燕,王华. 姜黄素对肝纤维化模型大鼠肝纤维化组织细胞因子的影响[J]. 医药导报, 2011, 30(4): 443-446. DOI: 10.3870/yydb.2011.04.010 |
| 作者姓名： | 张小斌 曾令兰 陈莎燕 王华 |
| 作者单位： | 1. 长江航运总医院、武汉脑科医院,430010 2. 华中科技大学同济医学院附属协和医院感染科,武汉,430022 |
| 摘 要： | 目的 了解姜黄素对实验大鼠肝纤维化组织中的基质蛋白酶-13(MMP-13)和金属蛋白组织抑制因子-(TIMP-1)的干预. 方法将22只雄性SD大鼠随机分为正常组(n=7)、模型组(n=6)和治疗组(n=9). 模型组和治疗组用四氯化碳制备大鼠肝纤维化模型,造模6周治疗组加用姜黄素,在10周末处死各组大鼠,取肝脏进行苏木精 伊红(hematoxylin and eosin,HE)染色,运用半定量逆转录聚合酶链反应(RT-PCR)方法检测MMP-13和TIMP-1的含量. 结果 HE染色病理分级明显,治疗组与其他两组比较,HE染色病理分级高于正常组,低于模型组,差异均有统计学意义(P<0.05);而MMP-13mRNA的表达组间变化不大,但TIMP-1mRNA的含量在各组间差异有统计学意义(P<0.05),模型组和治疗组TIMP-1表达水平高于正常组,治疗组的TIMP-1mRNA表达与模型组比较明显下降(P<0.05). 结论 姜黄素有抗纤维化作用,其机制可能是通过抑制大鼠肝脏中TIMP-1mRNA表达,减少胶原在肝脏内沉积,从而逆转和阻遏纤维化形成. |
| 关 键 词： | 姜黄素 肝纤维化 基质蛋白酶-13 金属蛋白酶组织抑制因子-1 |
| 收稿时间： | 2010-01-15 |
| 修稿时间： | 2010-05-16 |
Effects of Curcumin on the Expression of Cytokines in Livers of Rats with Fibrosis |
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| ZHANG Xiao-bin,ZENG Ling-lan,CHEN Sha-yan,WANG Hua. Effects of Curcumin on the Expression of Cytokines in Livers of Rats with Fibrosis[J]. Herald of Medicine, 2011, 30(4): 443-446. DOI: 10.3870/yydb.2011.04.010 | |
| Authors: | ZHANG Xiao-bin ZENG Ling-lan CHEN Sha-yan WANG Hua |
| Affiliation: | ZHANG Xiao-bin1,ZENG Ling-lan2,CHEN Sha-yan2,WANG Hua2(1.Changjiang River Shipping Hospital,Wuhan Cerebral Hospital,Wuhan 430010,China,2.Laboratory of Infections Diseases,Union Hospital Affiliated with the Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China) |
| Abstract: | Objective To observe the effects of curcumin on mRNA expression of matrix metal proteinase-13(MMP-13) and the tissue inhibitor of metal proteinase-1(TIMP-1) in hepatic tissues of experimental rats. Methods 22 male Sprague-Dawley rats were randomly divided into 3 groups : normal group(n=7),model group(n=6) and treatment group(n=9).Hepatic fibrosis was induced by CCl4 in rats,which were treated with treatment after 6 weeks in the treatment group.All rats were sacrificed by the end of the 10th week,when HE sta... |
| Keywords: | Curcumin Liver fibrosis Matrix metalloproteinase-13 Tissue inhibitors of metalloproteinase-1 |
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