P16INK4a和P14ARF蛋白在喉表达及临床意义鳞癌中的

【摘要】目的探讨P16INK4a和P14ARF蛋白在喉鳞状细胞癌组织中的表达及其临床意义。方法采用免疫组织化学EnVision法检测71例喉鳞状细胞癌组织中P16INK4a和P14ARF蛋白的表达。结果71例喉鳞状细胞癌标本中，35例（49．3％）P16INK4a蛋白阴性，29例（40．8％）P14ARF蛋白阴性，15例（21．1％）P16INK4a和P14ARF蛋白表达均阴性。P16INK4a和P14ARF蛋白阴性间无相关性（P〉O．05）。P16INK4a和P14ARF蛋白阴性率均与患者性别、年龄及临床分期无相关性（P〉0．05）。P14ARF蛋白在Ⅰ—Ⅱ期病例中强阳性占阳性比例为41．7％，在Ⅲ～Ⅳ期中强阳性比例为11．1％。结论P16INK4a和P14ARF在喉鳞状细胞癌发生过程中起重要作用，两种蛋白的失活是各自独立的事件。P14ARF蛋白的缺失发生在喉鳞状细胞癌早期。（中国眼耳鼻喉科杂志，2010，10：218—220）

Expression and significance of P16INK4a and P14ARF proteins in laryngeal squamous cell carcinoma

Objective To study the expression and clinical significance of P16INK4a and P14ARF proteins in laryn- geal squamous cell carcinoma. Methods The expression of P16INK4a and P14ARF proteins was detected in 71 carcinoma samples by immunohistochemistry （ EnVision method）. Results The negative expression of P16INK4a and P14ARF was de- tected in 35 samples（49.3 % ）and 29 sanlples（40.8 % ） respectively. While 15 cases（21.1% ）showed simultaneous loss in both proteins. Rank correlation analysis revealed that there was no correlation between negative expression of P16INK4a and P14ARF proteins in carcinoma（ P 〉 0.05 ）. The negative expression rate of P16INK4a and P14ARF was unrelated to the clinical data including gender, age and clinical stage. The overpositive proportion of all P14ARF positive cases in stage I to II was 41.7% and 11.1% in stage III to IV. Conclusions The aberrance of P16INK4a and P14ARF gene might be as- sociated with the oncogenesis of carcinoma. There was no correlation between negative expression of P16INK4a and P14ARF proteins in carcinoma. Moreover, the loss of P14ARF protein occurred in the early stage of carcinoma. （ Chin J Ophthalmol and Otorhinolaryngo1,2010,10：218-220）