| 紫球藻(Porphyridiumcruentum)原生质体分离研究 |
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| 引用本文： | 王明兹,庄惠如,陈必链,施巧琴,吴松刚. 紫球藻(Porphyridiumcruentum)原生质体分离研究[J]. 药物生物技术, 2001, 8(1): 26-29 |
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| 作者姓名： | 王明兹 庄惠如 陈必链 施巧琴 吴松刚 |
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| 作者单位： | 福建师范大学生物工程学院, 福州350007 |
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| 基金项目： | 福建省计委(1996 050号)、自然基金(B0020001)资助项目 |
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| 摘 要： | 收集对数生长期的紫球藻细胞,经1%氯化苄预处理后,用1.8%纤维素酶和1.2%果胶酶组成的混合水解酶液（含0.6mol/LNaCl,pH7.2）,在28℃下缓慢振荡酶水解细胞壁,4h后原生质体分离率比未预处理组提高74%,在最佳分离条件下的原生质体分离率高达56.4%,基本能满足原生质体融合的需求。
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| 关 键 词： | 紫球藻 原生质体 分离 氯化苄 |
| 文章编号： | 1005-8915(2001)04-0026-04 |
| 修稿时间： | 1999-12-16 |
Study on the ProtoplastsFormation from Porphyridium Cruentum |
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| Abstract: | The cells of Porphyridium cruentum cultured 4 days werepretreated with 1% benzyl chloride for 30 minutes , then moved into mixing enzyme solution of 1.8% cellulase and 1.2% pectinase (0.5 mol/L NaCl, pH 7.2) , and shaken gently (50 r/min)under 28℃. The protoplasts release rate amounts to 74% higher than non-pretreated ones 4hr latter . Under the best circumstance , the protop lasts release rate can be as high as 56.4%, which can be used on protoplast fusion. |
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| Keywords: | Porphyridium cruentum Protoplasts Released Benzyl choride |
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