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| Notch 3沉默对人急性T淋巴细胞白血病细胞增殖与凋亡的影响 | |
| 引用本文： | 项捷,欧阳永日,崔颖,林芳,任继鸿,龙敏,陈曦,卫军霞,张惠中. Notch 3沉默对人急性T淋巴细胞白血病细胞增殖与凋亡的影响[J]. 陕西肿瘤医学, 2011, 0(8): 1482-1487 |
| 作者姓名： | 项捷 欧阳永日 崔颖 林芳 任继鸿 龙敏 陈曦 卫军霞 张惠中 |
| 作者单位： | 第四军医大学唐都医院中心实验室,陕西西安710038 |
| 基金项目： | 国家自然科学基金资助项目（编号：30772181） |
| 摘 要： | 目的：探讨Notch 3受体特异性沉默对急性T淋巴细胞白血病的影响。方法：运用survivin启动子介导的RNA干涉方法特异性地封闭急性T淋巴细胞白血病细胞SupT1中Notch 3基因的表达。结果：survivin启动子介导的RNA干涉系统能特异而高效封闭肿瘤细胞内源Notch 3基因的表达,Notch 3基因表达的下调能够使肿瘤细胞的增殖活性降低,凋亡明显增加。结论：Notch 3基因沉默可有效抑制白血病细胞的增殖,并促进其凋亡,为急性T淋巴细胞白血病相关基因功能研究及靶向性治疗探索了一种新策略。 |
| 关 键 词： | Notch3 survivin启动子 急性T淋巴细胞白血病 |
Effect of Notch 3 silencing on apoptosis and proliferation of acute T-cell lymphoblastic leukemia cells |
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| XIANG Jie,OUYANG Yongri,CUI Ying,LIN Fang,REN Jihong,LONG Min,CHEN Xi,WEI Junxia,ZHANG Huizhong. Effect of Notch 3 silencing on apoptosis and proliferation of acute T-cell lymphoblastic leukemia cells[J]. Shaanxi Oncology Medicine, 2011, 0(8): 1482-1487 | |
| Authors: | XIANG Jie OUYANG Yongri CUI Ying LIN Fang REN Jihong LONG Min CHEN Xi WEI Junxia ZHANG Huizhong |
| Affiliation: | Department of Clinical Laboratory and Research Center,Tangdu Hospital,Fourth Military Medical University,Xi＇an 710038,China |
| Abstract: | Objective：To explore the effects of silencing of Notch 3 on the development of T-cell acute lymphoblastic leukemia.Methods： We investigated the effect of Notch 3 silencing on SupT1 cells using short hairpin RNA（shRNA） driven by survivin promoter.Results： shRNA targeting Notch 3 could down-regulate the expression of Notch 3 effectively.Down-regulating expression of Notch 3 correlated with a significant apoptosis and a proliferation inhibition.Conclusion： Notch silencing by shRNA driven by surviving promoter inhibits the proliferation and promotes the apoptosis of SupT1.This study provides an alternate therapy strategy against Notch signaling pathway. |
| Keywords: | Notch 3 survivin promoter T-ALL |
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