mir-27a、mir-200c及mir-145对肾癌细胞侵袭能力的影响

目的：探讨microRNA的表达在两种人肾癌细胞侵袭能力中的差异,为研究肾癌转移机制提供线索。方法：Transwell小室法检测两种人肾透明细胞癌细胞786-0、Caki-1侵袭能力差异。Real-time quantitativePCR方法检测两种细胞中mir-27a、mir-200c、mir-145表达水平。建立786-0及Caki-1细胞的裸鼠移植瘤模型,检测裸鼠786-0及Caki-1细胞移植瘤中mir-27a、mir-200c、mir-145可能调控的相应下游细胞黏附分子CD44、E-cadherin、β-catenin表达水平。结果：786-0细胞穿过Transwell小室的微孔膜的细胞数（196.8±4.9）明显高于Caki-1细胞（166.8±7.1）。786-0细胞中mir-27a、mir-200c、mir-145表达较Caki-1细胞明显升高。观察8周,786-0及Caki-1细胞在5×106/只的细胞数量下成瘤率为100%。CD44在裸鼠786-0细胞移植瘤中表达明显高于裸鼠Caki-1细胞移植瘤,β-catenin、E-cadherin在两种细胞裸鼠移植瘤中表达无明显差异。结论：mir-27a可能通过影响下游细胞黏附分子CD44的表达,影响肾癌细胞的侵袭能力,而mir-200c和mir-145影响肾癌侵袭能力的途径尚有待研究。

The influence of mir-27a,mir-200c and mir-145 on the invasion ability of renal cancer cells

Objective：To investigate the effect of microRNA expression on the invasion ability of renal cell carcinoma cells 786-0 and Caki-1,and to search for the metastatic mechanism of renal cell carcinoma.Methods： The difference in invasion ability of 786-0 and Caki-1 was evaluated by Transwell assay;the expressions of mir-27a,mir-200c,mir-145 were determined by real-time quantitative PCR;The nude mice xenograft models of 786-0 and Caki cells were established,and the exprssions of CD44,E-Cadherin,β-catenin which were regulated by mir-27a,mir-200c and mir-145 in the xenograft were detected by immunochemistry.Results： More 786-0 cells migrated through the PCS ester membrane compared with Caki-1 cells.The expressions of mir-27a,mir-200c and mir-145 in 786-0 cells were significantly elevated compared with Caki-1 cells.The tumor formation rate of 786-0 and Caki-1 cells at 5×106/number of cells was 100% by observing 8 weeks.The expression of CD44 protein which was affected by mir-27a in 786-0 xenograft was significantly elevated compared with Caki-1 xenograft,while the expressions of E-cadherin and β-catenin protein which were affected by mir-200c and mir-145 respectively in the two xenografts with no significant difference in the expression system.Conclusion： mir-27a in these two renal cancer cells may influence the expression of the downstream corresponding cellular adherin molecular CD44 which leads to the difference in invasion ability,while the mechanism of which mir-200c and mir-145 influence the difference in invasion ability between the two renal cancer cells is not clear yet.