RhG-CSF对乳腺癌细胞MCF-7增殖作用的影响

目的：探讨人重组粒细胞集落刺激因子（RhG-CSF）对乳腺癌细胞株MCF-7的增殖作用的影响。方法：体外培养人乳腺癌细胞株MCF-7,将含不同浓度RhG-CSF的培养液与MCF-7细胞共同培养不同时间,采用MTT比色法计算细胞生长增殖率;Anexxin V/PI双染试剂盒检测细胞凋亡率。实验结果用SPSS.v16.0软件分析。结果：不同浓度RhG-CSF处理细胞后,可以显著促进MCF-7细胞株的增殖（P〈0.01）,且当浓度为10μg/L时增殖作用最强。同时各浓度的RhG-CSF亦可抑制MCF-7细胞凋亡（P〈0.01）。结论：RhG-CSF可以显著促进人乳腺癌细胞株MCF-7增殖。当浓度小于10μg/L时,增殖呈浓度依赖性。大于10μg/L时,增殖能力反而逐渐下降。同时RhG-CSF亦可抑制MCF-7细胞凋亡。

RhG-CSF promotes proliferation of MCF-7 human breast cancer cells

Objective：To evaluate the effect of human recombinant colony-stimulating factors（RhG-CSF） in breast cancer cells.Methods： We cultured MCF-7 cells over a wide range of pharmacologic doses and hours,the MCF-7 cell growth curve was drawn by（MTT） assay.Flow cytometry（FCM） was adopted to analyze quantitatively the cell apoptosis.Results： As compared with MCF-7 cells cultured with DMEM,the cells cultured with RhG-CSF was markedly eugonic（P0.01）,and the largest eugonic effect was seen at 10μg/L.Moreover,apoptotic cells which cultured with RhG-CSF was also decreased（P0.01）.Conclusion： RhG-CSF can significantly promote MCF-7 cell proliferation.And the growth in a dose-dependent fashion when the dose less than 10μg/L,more than 10μg/L,it was contrary.