Arrangement and number of clustered regularly interspaced short palindromic repeat spacers are associated with erythromycin susceptibility in emm12, emm75 and emm 92 of group A streptococcus |
| |
| Affiliation: | 1. Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan;2. Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan;3. Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan;4. Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan;5. Department of Nursing, I-Shou University, Yanchao Campus, Kaohsiung, Taiwan;6. Department of Biochemistry, College of Medicine, National Cheng Kung University, Tainan, Taiwan;7. Department of Paediatrics, College of Medicine, National Cheng Kung University, Tainan, Taiwan;8. Centre of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan |
| |
| Abstract: | Clustered regularly interspaced short palindromic repeats (CRISPR) are composed of numerous repeat-spacer units and are considered a prokaryotic defence system against foreign nucleic acids. Since antibiotic-resistant genes are frequently encoded in foreign nucleic acids, the aim of this study was to test whether erythromycin susceptibility in group A streptococcus (<Streptococcus pyogenes) is associated with characteristics of CRISPR elements. Erythromycin susceptibility of 330 isolates collected between 1997 and 2003 was analysed. Among 29 emm types, emm12, emm75 and emm92 showed significant changes in erythromycin-resistance rates. By sequencing the spacers from two CRISPR loci, spacer contents in emm12, emm75 and emm92 strains were associated with erythromycin susceptibility. Strains with fewer spacers were more resistant to erythromycin. Moreover, in emm4 strains, which showed no significant change in their annual erythromycin-resistance rate, CRISPR type and number of spacers were not correlated with erythromycin susceptibility. These results highlight a novel association between CRISPR spacer content and erythromycin susceptibility in group A streptococcus. |
| |
| Keywords: | Clustered regularly interspaced short palindromic repeats epidemiology study erythromycin group A streptococcus macrolide |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
