神经再生素对乳鼠心肌细胞缺氧/复氧损伤的保护作用

目的:研究神经再生素(NRF)对纯化培养心肌细胞缺氧/复氧损伤的保护作用及机制。方法:采用纯化培养的心肌细胞建立缺氧/复氧损伤模型,观察心肌细胞形态学变化,测定细胞超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)和线粒体脱氢酶含量改变,探讨NRF对心肌细胞缺氧/复氧损伤的保护作用。结果:缺氧/复氧损伤后,胞体折光性下降,突起缩短或消失,搏动减弱或消失,NRF各剂量组心肌细胞形态均较缺氧/复氧组明显改善,NRF各剂量组SOD、MDA、线粒体脱氢酶活性与缺氧/复氧组比较差异有统计学意义(P<0.01)。结论:神经再生素具有明显的抗缺氧/复氧损伤,保护心肌作用,其机制可能与其抑制心肌脂质过氧化损伤有关。

Protective effects of never regeneration factor on hypoxia/reoxygenation injury in cultured neonatal rat myocardial cells

Objective:To study the protective effects of NRF on hypoxia/reoxygenation injury in cultured neonatal rat myocardial cells.Methods:With the hypoxia/reoxygenation injury model of cultured neonatal rat myocardial cell,the modality of the cells and the levels of the SOD,MDA,LDH,dehydrogenase in mitochondria were detected to study the effects of NRF on hypoxia/reoxygenation injury.Results:Refracting power of the cells declined and the beating became weak or even stopped in H/R group.Compared with the control group,the activities of SOD and dehydrogenase in mitochondria decreased(P<0.01)while the contents of LDH and MDA increased.Compared with H/R group,the activities of SOD and dehydrogenase in mitochondria increased(P<0.01) and the contents of LDH and MDA decreased significantly in each NRF group(P<0.01).Conclusion:NRF has protective effects on the cultured neonatal rat myocardial cells injured by hypoxia/reoxygenation.The mechanisms are related to its inhibition of lipid peroxidation.