高白细胞对血细胞分析仪检测红细胞和血红蛋白的影响

目的探讨高白细胞(WBC)(>70×109/L)对XE2100和LH750血细胞分析仪测定红细胞和血红蛋白的影响。方法采集65例WBC>70×109/L(19例WBC≥250×109/L、19例150×109/L70×109/L)组两种方法测定WBC差异无统计学意义(P>0.05),而RBC和Hb差异有统计学意义(P<0.05);对照组WBC、RBC和Hb检测结果均一致。线性回归提示WBC对两种方法测定RBC和Hb的差异具有正相关性的影响。结论 XE2100与LH750检测高WBC(>70×109/L)患者血样的RBC和Hb差异有统计学意义,临床应用应引起重视。

The influence of high WBC on detection of RBC and Hb by hematology analyzer

Objective To discuss the influence of high white blood cell（WBC） in determination of red blood cell（RBC） and hemoglobin（Hb） by Sysmex XE2100（XE2100） and Beckman Coulter LH750（LH750）.Methods We investigated the effect of high WBC on RBC and Hb reading by XE2100 and LH750,in 65 patients with WBC70×10^9/L（19 patients with WBC≥250×10^9/L,19 patients with 249.9×10^9/L〈WBC〈150×10^9/L and 27 patients with 149.9×10^9/L〈WBC〈70×10^9/L） and 63 control populations（WBC≤70×10^9/L）.Paired t Test,Bland-Altman plots and linear regression were investigated.Results There was no significant difference in WBC not only experiment group but controls,a poor agreement between the two methods of RBC and Hb was found in patients with a high WBC in comparison with that of the control population.Linear regression showed a concentration dependent influence of high WBC,RBC and Hb reading by the two methods.Conclusion The difference between XE2100 and LH750 assay methods is due to the high WBC,and the result of XE2100 is less influenced by the presence of a high WBC than that of LH750.