3种检测乙型肝炎病毒方法的比较及临床应用

目的探讨3种检测乙型肝炎病毒(HBV)方法的比较和临床应用。方法采用微粒子酶免疫分析(MEIA)法和酶联免疫吸附试验(ELISA)法检测血清HBV标志物,实时荧光定量聚合酶链反应(FQ-PCR)法检测HBV-DNA。结果 MEIA法与ELISA法检测HBV血清学结果的符合率较高,经χ2检验,两种测定方法所得结果差异无统计学意义(P>0.05);Ⅰ组的HBV-DNA阳性率显著高于其他组,差异具有统计学意义(P<0.05)。结论 MEIA法联合FQ-PCR法或者ELISA法联合FQ-PCR法应用,为临床提供HBV感染、复制及传染性的判断,对于疗效的分析具有重要价值。

Evaluation of three methods for testing hepatitis B virus and their clinical applications

Objective To evaluate the three methods for testing hepatitis B virus and their clinical application.Methods We detected the immunity markers of HBV-M（HBsAg,HBsAb,HBeAg,HBeAb,HBcAb） with MEIA and ELISA,and DNA content of HBV with FQ-PCR in order to do contrast analysis.Results Compared with paired χ^2 test,there was no significant statistical difference between the two results by MEIA and ELISA（P〈0.05）.The positive rate of the group（HBsAg,HBeAg,HBcAb） was the highest,significantly higher than the other groups（P〈0.05）,and the content of virus was the most.Conclusion Combined detection of HBV-M by MEIA or ELISA and HBV-DNA can help doctors to determine the infection and reproduction of HBV,and it can offer a credible proof to evaluate therapy effect of antiviral therapy.