| SYSMEX XE-5000血细胞分析仪血小板计数性能评价分析 |
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| 引用本文： | 刘纹,郑炘,刘晓敏. SYSMEX XE-5000血细胞分析仪血小板计数性能评价分析[J]. 检验医学与临床, 2011, 8(20): 2492-2494. DOI: 10.3969/j.issn.1672-9455.2011.20.030 |
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| 作者姓名： | 刘纹 郑炘 刘晓敏 |
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| 作者单位： | 中山大学肿瘤防治中心检验科,广州,510060 |
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| 摘 要： | 目的评价SYSMEX XE-5000全自动血细胞分析仪对血小板(PLT)检测结果的可靠性。方法取257份乙二胺四乙酸二钾(EDTA-K2)抗凝静脉全血标本,使用鞘流电阻抗法(impedance,PLT-I)进行血小板检测,根据血小板直方图、血小板平均体积(MPV)、红细胞平均体积(MCV)及血涂片法复查,按不同形态分组,分为正常血小板体积组、大血小板组、小红细胞组,分别用荧光法(optical,PLT-O)与显微镜目测法(microscopic,PLT-M)作对比试验。以及另取30份健康体检者的EDTA-K2抗凝静脉全血标本做红细胞碎片干扰试验,运用SPSS16.0及Excel对相关数据进行统计分析。结果正常体积血小板分布组3种方法之间结果无统计学差异;大血小板组PLT-I结果假性降低,PLT-I与PLT-M法结果差异有统计学意义,PLT-O与PLT-M法结果差异无统计学意义;小红细胞组PLT-I结果假性增高,PLT-I与PLT-M法结果差异有统计学意义,PLT-O与PLT-M法结果差异无统计学意义;RBC碎片干扰试验中,PLT-O对红细胞碎片显示出较强的抗干扰能力。结论正常血小板体积标本SYS-MEX XE-5000全自动血细胞分析仪常规使用PLT-I血小板计数结果正确可靠,但血小板直方图、MPV、MCV异常时标本仪器计数血小板不可靠,需与PLT-O或显微镜手工计数法相结合加以纠正。
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| 关 键 词： | SYSMEXXE-5000 血细胞分析仪 血小板计数 |
The performance evaluation of platelet counting in Sysmex XE-5000 |
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| LIU Wen,ZHENG Xin,LIU Xiao-min. The performance evaluation of platelet counting in Sysmex XE-5000[J]. Laboratory Medicine and Clinic, 2011, 8(20): 2492-2494. DOI: 10.3969/j.issn.1672-9455.2011.20.030 |
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| Authors: | LIU Wen ZHENG Xin LIU Xiao-min |
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| Affiliation: | LIU Wen,ZHENG Xin,LIU Xiao-min(Department of Clinical Laboratory,Cancer Therapy Center,Sun Yat-sen University,Guangzhou 510060,China) |
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| Abstract: | Objective To evaluate the platelet(PLT) counting performance quality of Sysmex XE-5000 automatic hematology analyzer.Methods 257 EDTA-K2 blood samples were counted by XE-5000 with electric impedance(PLT-I).According to PLT histogram,MPV and MCV,we separated the results to normal platelet,large platelet and microcytes from different PLT shape,and used electric impedance(PLT-I) and microscopic(PLT-M) methods to compare with them.Another blood samples from 60 healthy people were tested with RBC fragment interf... |
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| Keywords: | SYSMEX XE-5000 hematology analyzer platelet count |
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