| 兔阴茎海绵体平滑肌细胞的体外培养及其生物学特性 |
| |
| 引用本文： | 段永芳,刘继红,曹正国,章咏棠. 兔阴茎海绵体平滑肌细胞的体外培养及其生物学特性[J]. 中华男科学杂志, 2002, 8(3): 178-180 |
| |
| 作者姓名： | 段永芳 刘继红 曹正国 章咏棠 |
| |
| 作者单位： | 华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉,430030 |
| |
| 摘 要： | 目的 :研究体外培养的新西兰白兔阴茎海绵体平滑肌细胞的生物学特性。 方法 :采用组织块培养法 ,对兔阴茎海绵体平滑肌细胞进行活细胞观察 ,并测定其细胞生长曲线、贴壁率、细胞分裂指数。 结果 :①兔阴茎海绵体平滑肌细胞为梭形 ,呈长轴平行排列 ,具有明显的方向性 ;②体外贴壁快 ,生长迅速 ,体外培养的阴茎海绵体平滑肌细胞在合适的传代条件和比例下能够生存并保持其稳定的生物学特性。 结论 :体外培养的兔阴茎海绵体平滑肌细胞模型可用于检测某些药物对阴茎勃起的影响。
|
| 关 键 词： | 阴茎海绵体 平滑肌细胞 细胞培养,体外 兔 |
| 文章编号： | 1009-3591(2002)03-0178-03 |
| 修稿时间： | 2001-06-18 |
Biological Characteristics of Rabbit Corporal Smooth Muscle Cells Cultured in vitro |
| |
| Yong Fang DUAN,Ji Hong LIU,Zheng Guo CAO,Yong Tang ZHANG. Biological Characteristics of Rabbit Corporal Smooth Muscle Cells Cultured in vitro[J]. National journal of andrology, 2002, 8(3): 178-180 |
| |
| Authors: | Yong Fang DUAN Ji Hong LIU Zheng Guo CAO Yong Tang ZHANG |
| |
| Affiliation: | Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. |
| |
| Abstract: | OBJECTIVES: To study the biological characteristics of rabbit corporal smooth muscle cells (SMC) cultured in vitro. METHODS: In vitro tissue culture technique, morphological observation, cell counting, mitosis index and adhesion rate evaluation were applied to study the biological features of the SMC. RESULTS: 1. SMC were spindle-shaped and parallel along their longitudinal axis, showing obvious orientation. 2. The attachment and the proliferation of SMC in vitro were rapid. SMC cultured in vitro can grow and maintain their steady characteristics provided appropriate passage rate and culture condition. CONCLUSIONS: The SMC cultured in vitro are proved to be used to evaluate and investigate the effect of some medicine on penile erection. |
| |
| Keywords: | Corpus cavernosum Smooth muscle cells Cell culture in vitro Rabbit |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
