| 尿中尼古丁含量的高效液相色谱测定法 |
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| 引用本文： | 冯斌,邵华,单永乐. 尿中尼古丁含量的高效液相色谱测定法[J]. 职业与健康, 2010, 26(14): 1586-1587 |
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| 作者姓名： | 冯斌 邵华 单永乐 |
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| 作者单位： | 山东省职业卫生与职业病防治研究院检测与评价中心,250062 |
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| 基金项目： | 山东省自然科学基金项目 |
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| 摘 要： | 目的建立一种实用的高效液相色谱法测定人尿中尼古丁含量的方法。方法在流动相为0.02mol/LH3PO4-KH2PO4(pH值=3)、流速为1.0ml/min、C18反相柱、室温、波长为262nm的条件下,测定尼古丁。结果标准曲线的相关系数0.9992,加标回收率大于91.5%,变异系数小于6.0%。结论应用该方法测定尿中尼古丁的含量,方法简便、准确、灵敏度高。
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| 关 键 词： | 高效液相色谱法 尼古丁 检测 尿液 |
Determination of Nicotine in Urine by HPLC |
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| FENG Bin,SHAO Hua,SHAN Yong-le. Determination of Nicotine in Urine by HPLC[J]. Occupation and Health, 2010, 26(14): 1586-1587 |
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| Authors: | FENG Bin SHAO Hua SHAN Yong-le |
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| Affiliation: | ( Shandong Institute for Occupational Health and Occupational Disease,250062,China) |
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| Abstract: | [Objective]To establish a method for determination of Nicotine in the urine by high performance liquid chromatography（HPLC）.[Methods]Under the condition of mobile phase 0.02 mol/L H3PO4-KH2PO4（pH=3）,velocity of flow 1.0 ml/min,C18 reversed phase column,room temperate,Wavelength 262 nm,the nicotine was determined.[Results]The correlation coefficient of standard curve was more than 0.9992,and the recovery was more than 91.5%,the coefficient of variation was less than 6.0%.[Conclusion]The method is simple,accurate,and sensitive for nicotine determination in urine. |
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| Keywords: | High performance liquid chromatography（HPLC） Nicotine Determination Urine |
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