阴离子交换树脂纯化三七发酵产物中总皂苷的工艺优选

目的:为了得到高纯度的三七总皂苷,利用阴离子交换树脂对三七发酵产物进行纯化,优选纯化工艺参数。方法:选用三七发酵产物为研究对象,以总皂苷含量或纯度为指标,采用单因素试验考察树脂型号、径高比、上样液质量浓度、上样流速、吸附时间、洗脱剂种类、洗脱液用量、洗脱流速、上样液p H等条件对洗脱液中总皂苷含量的影响。结果:选取D301型阴离子树脂,径高比1∶8.3,上样液质量浓度0.25 g·m L~(-1),树脂干重-生药量(1∶0.64),上样流速2 BV·h~(-1),上样液p H 9,吸附时间15 min,洗脱流速2 BV·h~(-1),加水5 BV洗脱杂质,加60%乙醇4 BV对目标成分进行洗脱。三七发酵产物中总皂苷纯度90%。结论:利用阴离子交换树脂纯化三七发酵产物中总皂苷的方法简便可行,纯化效果好,能满足于工业生产的要求,为该有效部位的新药及相关产品开发提供参考。

Optimization of Purification Process for Total Saponins in Fermentation Products of Notoginseng Radix et Rhizoma by Anion Exchange Resin

Objective: To optimize purification process of total saponins in fermentation products of Notoginseng Radix et Rhizoma by anion exchange resin. Method: Single factor tests were conducted on resin type,diameter-height ratio of resin column,flow rate,absorption-influencing factors and elution-influencing factors,in order to ensure purity of total saponins in fermentation products of Notoginseng Radix et Rhizoma. Result: D301 anion resin was selected,its optimum purification parameters were as follows:diameter-height ratio of 1:8.3,dry weight of resin-fermentation products of 1:0.64,sample solution concentration of 0.25 g·mL^-1,sample solution pH of 9,flow rate of 2 BV·h^-1,adsorption time of 15 minutes.In addition,impurities were removed by 5 BV of distilled water before 4 BV of 60% ethanol was used to elute target compounds.Purity of total saponins had been successfully purified to more than 90%. Conclusion: This study achieves a simple,efficient and repeatable process with excellent purification performances for total saponins in fermentation products of Notoginseng Radix et Rhizoma,based on which industrial production can be fulfilled.It provides a theoretical support for new drug and related products development of total saponins in fermentation products of Notoginseng Radix et Rhizoma.