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| Touchdown PCR检测野生中缅树鼩伯氏疏螺旋体感染 | |
| 引用本文： | 柳爱华,程玲,张才军,陈芳,施明,陈亮,沈培清,刘汝文,角建林,李玛琳,宝福凯.Touchdown PCR检测野生中缅树鼩伯氏疏螺旋体感染[J].中国热带医学,2009,9(4):621-622,734. |
| 作者姓名： | 柳爱华 程玲 张才军 陈芳 施明 陈亮 沈培清 刘汝文 角建林 李玛琳 宝福凯 |
| 作者单位： | 1. 昆明医学院实验动物质量检测站,云南,昆明,650031;昆明医学院生物化学与分子生物学教研室,云南,昆明,650031 2. 昆明医学院生物化学与分子生物学教研室,云南,昆明,650031 3. 昆明医学院实验动物质量检测站,云南,昆明,650031;昆明医学院微生物学与免疫学教研室,云南,昆明,650031 4. 昆明医学院实验动物质量检测站,云南,昆明,650031 |
| 基金项目： | 云南省科技计划项目平台项目(No.2006PT07-1) |
| 摘 要： | 目的用Touchdown PCR检测野生中缅树鼩的伯氏疏螺旋体感染。方法从昆明及其周边地区捕获30只野生中缅树鼩，乙醚深度麻醉后处死，取内脏-30℃保存备用。用总DNA提取试剂盒提取组织总DNA，用紫外可见光分光光度计测定样品的DNA含量，用热启动Touchdown PCR技术检测野生中缅树鼩的伯氏疏螺旋体flaB基因。对PCR产物做凝胶电泳，凝胶成像仪观察并照相。结果Touchdown PCR有良好的特异性和敏感性。实验重复3次，结果一致，野生树鼩的伯氏疏螺旋体DNA阳性率为63．33％（19／30）。结论野生树鼩存在伯氏疏螺旋体感染，并且感染率较高。 |
| 关 键 词： | 树鼩 伯氏疏螺旋体 Touchdown PCR 莱姆病 |
Detection of Borrelia burgdorferi in wild tree shrews by touchdown PCR |
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| LIU Ai-hua,CHENG Lin,ZHANG Cai- jun,et al..Detection of Borrelia burgdorferi in wild tree shrews by touchdown PCR[J].China Tropical Medicine,2009,9(4):621-622,734. | |
| Authors: | LIU Ai-hua CHENG Lin ZHANG Cai- jun |
| Institution: | 1.Quality Control Station for Experimental Animal;Kunming Medical College;Kunming 650031;Yunnan;P.R.China |
| Abstract: | Objective To investigate the status of Borrelia burgdorferi infection in wild tree shrews(Tupaia belangeri) in Yunnan Province.Methods There 30 wild tree shrews were captured in Kunming and its suburban areas.Animals were anesthetized with overdosed ethoxyethane,3-oxapentane and sacrificed by euthanasia,their internal organs were collected and frozen in icebox at-30oC before use.Total DNA samples were extracted from tissues and determined by UV spectrometer.Touchdown PCR were performed to detect flab gene o... |
| Keywords: | Tree shrew Borrelia burgdorferi Touchdown PCR Lyme disease |
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