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| 钛颗粒对小鼠骨髓间充质干细胞生物学行为影响的体外研究 | |
| 引用本文： | 程伟,孟波. 钛颗粒对小鼠骨髓间充质干细胞生物学行为影响的体外研究[J]. 中国口腔种植学杂志, 2010, 15(2): 51-54 |
| 作者姓名： | 程伟 孟波 |
| 作者单位： | 1. 南京市口腔医院口腔种植科,南京大学附属口腔医院,210008 2. 南方医科大学附属口腔医院,广东省口腔医院,510280 |
| 摘 要： | 目的：研究钛颗粒对小鼠骨髓间充质干细胞生物学行为的影响。方法：将钛颗粒脱内毒素处理后,与小鼠BMSC共培养,检测钛颗粒对它们存活率、增殖率的影响,并通过透射电镜检测细胞对钛颗粒的吞噬情况。此外,对细胞骨架F-actin进行荧光染色,观察钛颗粒对BMSC细胞骨架的影响。结果：钛颗粒降低了BMSC的生存率并抑制其增殖活性,该抑制作用随钛颗粒浓度的增加而升高。BMSC可吞噬钛颗粒,而且钛颗粒可影响BMSC的正常细胞骨架。结论：钛颗粒对BMSC具有抑制作用,促进了种植体周无菌性骨吸收。 |
| 关 键 词： | 钛颗粒 间充质干细胞 无菌性松动 吞噬 骨整合 |
The research of titanium particles on the behavior of mouse bone mesenchymal stem cells in vitro |
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| CHENG Wei,MENG Bo. The research of titanium particles on the behavior of mouse bone mesenchymal stem cells in vitro[J]. Chinese Journal of Oral Implantology, 2010, 15(2): 51-54 | |
| Authors: | CHENG Wei MENG Bo |
| Affiliation: | CHENG Wei,MENG Bo Nanjing Stomatological Hospital,Nanjing Unviersity,Nanjing,210008 Guangdong Provincial Stomatological Hospital,Guangzhou,510280 |
| Abstract: | Objective: To observe the effect of titanium particles on bone mesenchymal stem cell (BMSC) in vitro.Methods: BMSC were co-cultured with different concentrations of titanium particles.Cellular responses to the titanium particles were evaluated by changes in cell viability and cell proliferation.Moreover,the cytoskeleton of cells were evaluated and transmission electron microscopy (TEM) was also tested to examine the phagocytosis of titanium particles.Results: The results showed that BMSC co-cultured with ti... |
| Keywords: | titanium particles BMSC aseptic loosening phagocytosis osseointegration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |