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| 抗纤灵对5/6肾切除大鼠骨髓来源的成纤维细胞表型转化的影响 | |
| 引用本文： | 王东,张江,陈刚,何立群.抗纤灵对5/6肾切除大鼠骨髓来源的成纤维细胞表型转化的影响[J].中国中医药信息杂志,2012(9):33-35. |
| 作者姓名： | 王东 张江 陈刚 何立群 |
| 作者单位： | 1. 上海中医药大学附属曙光医院,上海200021 2. 合肥师范学院,安徽合肥230601 3. 上海市中医临床重点实验室,上海200021 4. 上海市高校中医内科 E-研究院,上海200021 |
| 基金项目： | 国家自然科学基金(81173219);科技部中医药行业科研专项(201007005);高等学校博士学科点专项科研基金(20093107110006);上海市科委创新行动计划(11DZ1973100);上海市教育委员会E-研究院建设计划(E03008);上海高校创新团队建设项目(200908) |
| 摘 要： | 目的观察抗纤灵对5/6肾切除大鼠骨髓来源的成纤维细胞表型转化的影响,探讨其抗肾纤维化的作用机制。方法将SD大鼠随机分为假手术组和造模组,造模组以5/6肾切除方法建立慢性肾衰竭动物模型,1周后按血肌酐水平将造模大鼠分为模型组、福辛普利组和抗纤灵组。各给药组给予相应药物灌胃,模型组和假手术组给予等量生理盐水灌胃,8周后采用实时荧光定量PCR和蛋白免疫印迹法测定各组大鼠肾组织α-平滑肌肌动蛋白（α-SMA）、Ⅰ型胶原和CD45基因及蛋白的表达。结果模型组大鼠表达α-SMA mRNA、Ⅰ型胶原mRNA和CD45mRNA水平较假手术组明显升高（P〈0.001或P〈0.05）,抗纤灵组与模型组比较均明显下降（P〈0.01或P〈0.05）;模型组大鼠表达α-SMA、Ⅰ型胶原和CD45蛋白水平较假手术组明显升高（P〈0.001）,抗纤灵组与模型组比较均显著下降（P〈0.001）。结论抗纤灵可降低Ⅰ型胶原和CD45的表达,抑制骨髓来源的成纤维细胞表型转化,从而延缓肾纤维化进程,保护残肾功能。 |
| 关 键 词： | 抗纤灵 α-平滑肌肌动蛋白 Ⅰ型胶原 CD45蛋白 表型转化 |
Effect of Kangxianling on Phaenotype Transformation of Bone Marrow-derived Fibroblast in 5/6 Nephrectomy Rats |
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| WANG Dong,ZHANG Jiang,CHEN Gang,HE Li-qun.Effect of Kangxianling on Phaenotype Transformation of Bone Marrow-derived Fibroblast in 5/6 Nephrectomy Rats[J].Chinese Journal of Information on Traditional Chinese Medicine,2012(9):33-35. | |
| Authors: | WANG Dong ZHANG Jiang CHEN Gang HE Li-qun |
| Institution: | 1,3,4 (1.Shuguang Hospital, Shanghai University of TCM,Shanghai 200021,China;2.Hefei Normal College,Hefei 230601,China;3.Shanghai Clinical Key Laboratory of TCM,Shanghai 200021,China;4.TCM Internal Medicine E-Institute of Shanghai University,Shanghai 200021,China) |
| Abstract: | Objective To observe the effect of Kangxianling on phaenotype transformation of bone marrow-derived fibroblast in 5/6 nephrectomy rats,and to explore its mechanism of anti-renal fibrosis.Methods SD rats were randomly divided into sham operation group and model group.The model group was made rat model of chronic renal failure with 5/6 nephrectomy.One week later,the model group rats were randomly divided into untreated group,fosinopril group and Kangxianling group by the serum creatinine level.The fosinopril group was given 3.3 mg/(kg.d) fosinopril,Kangxianling group was given 23 g/(kg.d) Kangxianling untreated group and sham operation group were given normal saline by gavage for 8 weeks.The expression of α-SMA,collagen Ⅰ and CD45 in the renal tissue were determined by real-time quantitative PCR(RT-PCR) and protein immunoblot method(Western blot).Results Compared with the sham operation group,the expression of α-SMA mRNA,collagen Ⅰ mRNA and CD45 mRNA levels in the model group were significantly higher(P <0.001 or P <0.05),and those in Kangxianling group were significantly decreased(P <0.01 or P <0.05).The expression of α-SMA,collagen Ⅰ and CD45 levels in the model group were significantly higher than that in the sham operation group,and those in Kangxianling group were significantly decreased(P <0.001).Conclusion Kangxianling could depress the expression of collagen Ⅰ and CD45,suppress phaenotype transformation of bone marrow-derived fibroblast,thereby delay the process of renal fibrosis and protect residual renal function. |
| Keywords: | Kangxianling α-smooth muscle actin collagen Ⅰ CD45 phaenotype transformation |
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