HPLC同时测定陇中损伤胶囊中3种皂苷含量

目的建立同时测定陇中损伤胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1含量的高效液相色谱梯度洗脱方法。方法采用C18柱（250 mm×4.6 mm,5μm）;流动相为乙腈（A）-0.05%磷酸溶液（B）;梯度洗脱0～32 min（A23%～23%）,32～42 min（A 23%～30%）,42～67 min（A 30%～46%）;检测波长203 nm;流速1.0 mL/min。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1进样量分别在1.02～8.50μg（r=0.9995）、0.90～7.50μg（r=0.9990）、1.17～9.75μg（r=0.999 6）范围内与峰面积线性关系良好,平均加样回收率为100.98%（RSD=2.71%）、97.73%（RSD=1.98%）、99.57%（RSD=1.62%）。结论所用方法简单易行、准确可靠,可作为陇中损伤胶囊的质量控制方法。

Simultaneously Determination of Three Kinds of Ginsenosides in Longzhong Sunshang Capsule by HPLC

Objective To establish the method for simultaneous determination of panax notoginseng saponins R 1,ginseng saponins Rg 1,ginseng saponins Rb 1 in Longzhong Sunshang Capsule by HPLC with gradient elution.Methods C 18 column(250 nm×4.6 nm,5 μm) was used with mobile phase of acetonitrile(A)-0.05% phosphoric acid solution(B),gradient elution 0-32 min(A 23%-23%),32-42 min(A 23%30%),42-67 min(A 30%-46%).The detection wavelength was 203 nm,and the flow rate was 1.0 mL/min.Results The linear relationship of panax notoginseng saponins R 1,ginseng saponins Rg 1,ginseng saponins Rb 1 was in the range of 1.02-8.50 μg(r =0.999 5),0.90-7.50 μg(r =0.999 0) and 1.17-9.75 μg(r = 0.999 6) respectively.The average recovery was 100.98%(RSD=2.71%),97.73%(RSD=1.98%) and 99.57%(RSD=1.62%).Conclusion The method is simple,accurate,reliable,and can be used for quality control of Longzhong Sunshang Capsule.