气相色谱法同时测定三川丹皮酚膏中4种成分含量

目的建立同步测定三川丹皮酚膏中樟脑、薄荷脑、龙脑和丹皮酚4种成分含量的气相色谱法。方法使用气相色谱仪,ZB-WAX毛细管柱（30 m×0.25 mm×0.25μm）。以氮气为载气,氢火焰离子化检测器,采用程序升温,外标法测定含量。结果三川丹皮酚膏中的樟脑、薄荷脑、龙脑和丹皮酚等4种物质在规定范围内有良好的线性关系,平均加样回收率分别为99.82%、99.28%、98.72%、97.35%。结论本方法简便,灵敏度高,重复性好,结果准确,适合于三川丹皮酚膏中樟脑、薄荷脑、龙脑和丹皮酚的含量测定。

Simultaneous Quantification of Four Compounds in Sanchuan Paeonol Ointment by GC

Objective To establish an efficacious gas chromatography(GC) method for the determination of camphor,menthol,borneol and paeonol in Sanchuan paeonol ointment.Methods The GC method was applied and the separation was performed by means of temperature programming on ZB-WAX capillary column(30 m×0.25 mm×0.25 μm).The analysis was carried out by external standard method on FID detector with nitrogen as the carrier gas.Results Camphor,menthol,borneol and paeonol in the ointment had a good linear relationship within the specific range,and the average recovery was 99.82%,99.28%,98.72% and 97.35%,respectively.Conclusion The method is simple and accurate,with good reproducibility and sensitivity,therefore can be used as a quality control method for the quantitative determination of camphor,menthol,borneol and paeonol in Sanchuan paeonol ointment.