免疫组化染色在前列腺穿刺活检中的应用

目的探讨免疫组化染色在前列腺穿刺活检中的应用价值。方法在组织学观察基础上,对321例前列腺穿刺标本进行P504s、P63、CK34βE12免疫组化检测。结果P504s在前列腺癌、前列腺上皮内瘤变(prostatic intraepithelial neoplasia,PIN)和良性增生组织中的阳性表达差异有统计学意义(P<0.01);在不同Gleason评分的前列腺癌组织中,P504s阳性表达差异无统计学意义(P>0.05);P63、CK34βE12在前列腺癌组织中表达呈阴性,在上皮内瘤变中呈不连续表达,在良性增生病变中呈强阳性表达。结论P504s是前列腺癌敏感而特异性标记物,与P63、CK34βE12联合标记在前列腺病变鉴别诊断中具有重要意义,可提高前列腺癌的诊断准确率。

The Application of Immunohistochemical Staining in Prostate Needle Biopsies

Objective To investigate the value of immunohistochemical staining in the diagnosis of prostate adenocarcinoma.Methods The 321 prostate needle biopsies had been diagnosed histopathologically.The immunohistochemical staining was performed to detect the expression of P504s,P63 and CK34βE12.Results The expression of P504s was significantly different among prostate adencarcinoma,high-grade prostatic intraepithelial neoplasia and benign prostatic hyperplasia(P<0.01),but was not significantly different among from prostate adencarcinoma of different Gleason grades(P>0.05).P63 and CK34βE12 were negative in prostate adenocarcinoma,interruptedly expressed in high-grade prostatic intraepithelial neoplasia and strongly positive in benign prostatic hyperplasia.Conclusion P504s is a sensitive and specific marker for prostate carcinoma.Combined detection of P63、CK34βE12、P504s has great significance in differential diagnosis of prostatic diseases.It can improve the accuracy of diagnosis in prostate carcinoma.