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| 车前草中熊果酸、齐墩果酸的HPLC测定 | |
| 引用本文： | 袁珂,李根林,李俊芝,杨怡. 车前草中熊果酸、齐墩果酸的HPLC测定[J]. 医学教育探索, 1999, 0(12): 901-903 |
| 作者姓名： | 袁珂 李根林 李俊芝 杨怡 |
| 作者单位： | 河南中医学院!郑州450003 |
| 基金项目： | 河南省自然科学基金!第9940240号 |
| 摘 要： | 采用ＨＰＬＣ法同时测定车前草中熊果酸、齐墩果酸的含量。选用Ｃ１８ＯＤＳ色谱柱，流动相：甲醇－水（８８：１２），检测波长：２２０ｎｍ，流速；０．６ｍＬ／ｍｉｎ，柱温：２５℃。熊果酸和齐墩果酸分别在０．４０４２～２．０２０μｇ（４＝０．９９８４）和０．４１０～２。０５０μｇ（ｒ＝０．９９９７）范围内呈线性；熊果酸、齐墩果酸的平均回收率分别为９８．２％（ＲＳＤ＝１．７４％）９７．８％（ＲＳＤ＝／２．２４ |
| 关 键 词： | 车前草 熊果酸 齐墩果酸 高效液相色谱 |
Determination of Ursolic Acid and Oleanolic Acid in Asiatic Plantain(Plantago asiatica) by HPLC |
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| Yuan Ke,Li Genlin,Li Junzhi,et al.. Determination of Ursolic Acid and Oleanolic Acid in Asiatic Plantain(Plantago asiatica) by HPLC[J]. Researches in Medical Education, 1999, 0(12): 901-903 | |
| Authors: | Yuan Ke Li Genlin Li Junzhi et al. |
| Abstract: | The contents of ursolic acid and oleanolic acid in Plantago asiatica L. was determined by HPLC. The HPLC system consisted of C_18-ODS, mobile phase of methanol-water (96: 4) mixture, with detection at 220 um, flow rate 0. 6 mL/min and column temperature 25 C. The linear ranges of determination of ursolic acid and oleanolic acid were 0. 404-- 2. 020 ug, r=0. 998 4 and 0. 410-2. 050 ug, r= 0. 999 7, respectively. The mean recovery of 98. 2 %, RSD 1. 74 %, for ursolic acid and 97. 8%, RSD 2. 24% for oleanolic acid is obtained. The method is sensitive, rapid and accurate with good reproducibility. |
| Keywords: | Plantago major L. ursolic acid oleanolic acid |
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