Experimental Study on Inhibition of S180 Tumor Cells by Agrimonia Pilosa Extract

We evaluated the in vitro and in vivo inhibition of S₁₈₀ tumor cell growth of the water extract of Agrimonia pilosa. Inhibitory effect of Agrimonia pilosa on in vitro-cultured S₁₈₀ cell proliferation was measured by MTT assay; mice model of transplanted tumor was established, after 8 days of continuous administration, the tumors were removed, weighed, and compared with the control group, and the in vivo anti-tumor effect of Agrimonia pilosa on mouse sarcoma S₁₈₀ was compared using inhibition rate. The in vitro anti-tumor experiment indicated that the inhibition rate gradually increases with the increase of extract concentration and the extension of time, with IC50 175.64, 90.59, and 71.74 µg/ml at 24 h, 4 h, and 72 h respectively. In this study, the in vitro MTT assay was used to determine the inhibitory effect of Agrimony pilosa water extract on S₁₈₀ tumor cells, the method is simple, reliable, and practical; mice model of in vivo transplanted S₁₈₀ tumor was established, which allowed direct observation of tumor inhibitory effect, and thus found out that the water extract of Agrimonia pilosa has inhibitory effect on S₁₈₀ tumor cells.