针刺对去卵巢大鼠骨折模型脑源性神经营养因子表达的影响

目的:观察针刺疗法对去卵巢大鼠骨折的效应及作用机制.方法:SD大鼠60只,随机分为4组:A组为正常对照组,B组为模型组,C组为针刺组,D组为尼尔雌醇组,除A组为假手术外,其余各组大鼠行"去卵巢手术",以建立骨质疏松症动物模型;术后3个月,4组动物均于左侧股骨中段建立闭合骨折模型,然后,各组分别按既定方案进行治疗处置:A组及B组每周每只大鼠灌服0.9%生理盐水3 mL;C组针刺左后肢的"环跳""后三里""阳陵泉""委中",每天1次;D组每只大鼠予尼尔雌醇溶液0.6 mL/100 g体重灌胃(尼尔雌醇浓度:0.2 mg/mL),每周1次.分别于骨折造模术后第7、14、21和28 d处死取材,进行组织学、脑源性神经营养因子(BDNF)和酪氨酸蛋白激酶B(TrkB)免疫组化染色检测.结果:骨痂HE染色光镜观察结果显示,A组、C组和D组的骨折愈合进程快于B组;各组骨痂均有BDNF、TrkB表达,时间主要在骨折后7 d和14 d,表达水平由高到低依次是A、C、D、B组.结论:骨质疏松性骨折的骨痂中BDNF、TrkB的表达减少,而针刺治疗能增加其表达,加快骨折愈合进程.

Effects of acupuncture on the expression of brain-derived neurotrophic factor in the ovariectomized rat fracture model

Objective To observe the effect of acupuncture on fracture in the ovariectomized rat and the mechanism. Methods Sixty SD female rats were randomly divided into 4 groups: normal group (group A), model group (group B) , acupuncture group (group C) and Nilestriol group (group D). In all the groups, except the group A which received sham operation, the rats were overiectomized for preparing the osteoporosis model. Three months after the ovariectomy, the left femurs of the rats were closely fractured. Then, the group A and B were treated with oral administration of normal saline solution, 3 mL, weekly. The rats in the group C were treated daily with acupuncture at "Huantiao"(GB 30), "Housanli"(ST 36), "Yanglingquan"(GB 34) and "Weizhong"(BL 40) on the left hind legs; the rats in the group D were given orally Nilestriol solution (0. 2 mg/mL) in a dose of 0. 6 mL/100 g body weight, weekly. At the 7~(th), 14~(th), 21~(st) and 28~(th) days, some rats were sacrificed and their fractural callus and blood samples were taken for histological examinations and immunohistochemical examination of brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB). Results HE stained callus slides observed by optical microscope showed that the process of fracture healing in the group A, C, D was faster than that in the group B. Positive im-muno-staining of BDNF and TrkB could be seen in the all groups, mainly on the 7 and 14 days after the fracture. The expression levels from high to low in turn were group A, C, D and B. Conclusion Expressions of BDNF and TrkB in callus of osteoporotic fracture were less than that of the normal fractures acupuncture can elevate the expression levels and accelerate the process of fracture healing.