桑黄胞内多糖抗肿瘤效应的实验研究

背景与目的:探讨桑黄胞内多糖的抗肿瘤和增效作用以及对S180荷瘤小鼠免疫功能的影响,为开发桑黄胞内多糖提供理论依据.材料与方法:采用S180荷瘤小鼠,随机分为正常对照组,荷瘤对照组,环磷酰胺(CP)组,桑黄胞内多糖低、中、高剂量组(50、100、200 mg/kg)和CP与桑黄胞内多糖低、中、高剂量联合用药组共9组,每组10只.分别观察桑黄胞内多糖及与CP联合用药后的抑瘤效果及对荷瘤小鼠免疫功能的影响,采用MTT法测定对S180荷瘤小鼠脾淋巴细胞转化能力的影响.结果:200、100 mg/kg剂量的桑黄胞内多糖对S180的抑制率分别为57.02%和61.40%;与环磷酰胺联合使用后,100 mg/kg的剂量抑制率可达79.51%;联合用药组荷瘤小鼠的胸腺指数和脾脏指数明显增加(P＜0.05),显示其对环磷酰胺造成的免疫器官损伤有明显的保护作用;桑黄胞内多糖组及其与CP联合用药组白细胞数(OD值)均较荷瘤组和CP组高(P＜0.05或P＜0.01),显示桑黄胞内多糖对CP造成的白细胞数下降有明显的拮抗作用,具有促进机体白细胞生成能力;桑黄胞内多糖对S180荷瘤小鼠脾淋巴细胞的转化具有明显的促进作用.结论:桑黄胞内多糖有明显的抗肿瘤作用,明显增强荷瘤小鼠免疫功能,并能降低化疗药物造成的免疫系统损伤,对环磷酰胺起增效减毒作用.

Experimental Study on Anti-tumor Effects of Intracellular Polysaccharide from Phellinus Linteus

BACKGROUND & AIM: To study the anti-tumor effects, efficacy enhancement and immunoregulatory function of intracellular polysaccharide(IPS) from liquid state fermentation of Phellinus linteus in S180-bearing mouse and provide evidence for exploring IPS. MATERIALS AND METHODS: S180-bearing mice were divided into 9 groups (10 mice per group) which were normal group, tumour-bearing group, Cyclophosphamide(CP) group, IPS (50, 100, 200 mg/kg) groups and co-administration groups of IPS(50,100,200 mg/kg) with CP. The anti-tumor effects and the influences on immunity function of both IPS and co-administration of IPS with CP were observed. The MTT method was used to measure the proliferation activity of splenic lymphocytes of S180-bearing mouse. RESULTS: At doses of 200 and 100 mg/kg, inhibition rates of S180 sarcoma were 57.02% and 61.40% respectively. The inhibition rate of co-administration of 100 mg/kg IPS with CP could reach 79.51%. IPS significantly increased thymus index and spleen index of S180-bearing mouse and protect the immune organs aginst CP. IPS promoted the growth of leucocytes and inhibited its reduction induced by CP. Besides, IPS enhanced cell-mediated immunity by accelerating T-cell proliferation. CONCLUSION: IPS of Phellinus linteus was shown to have significant anti-tumor effects and promoted the immune function of tumor-bearing mice. IPS could also mitigate the toxic and side effects of chemotherapeutic agents and enhancing the efficacy of CP.