环磷酰胺及塞替派诱导的人支气管上皮恶性转化细胞的细胞周期相关蛋白表达

目的　了解细胞周期调控系统各正、负调节因子在细胞发生恶性转化过程中的变化情况。方法　采用免疫组化技术分别检测了经环磷酰胺(CP)和塞替派 (TEPA)诱导永生化人支气管上皮细胞 (BEAS 2B)发生恶性转化的BEAS CP和BEAS TE细胞中P5 3、P16、P15、细胞周期蛋白 (cyclin)D1和RB蛋白的表达。结果　BEAS 2B细胞中表达阳性的P16、P15及RB蛋白 ,在BEAS CP和BEAS TE细胞中表达缺失 ;BEAS 2B细胞中检测呈阴性的P5 3蛋白 ,在BEAS CP和BEAS TE细胞中呈阳性 ;cyclinD1蛋白在 3株细胞中检测均为阳性。结论　转化细胞中P16、P15及RB蛋白的表达异常可能影响了细胞周期中P16 (P15 ) /cyclinD1 CDK4 /pRB调控途径的自稳平衡。两转化细胞P5 3蛋白的阳性表达提示转化细胞P5 3蛋白的功能已发生改变。

Expression of cell cycle regulators in human bronchial epithelial cells malignantly transformed by cyclophosphamide and thiotepa

AIM Human bronchial epithelial cells(BEAS-2B) transformed by cyclophosphamide(CP)(BEAS-CP) and thiotepa(TEPA)(BEAS-TE) were used to study alterations of cell cycle regulated proteins and their role in the processes of cell transformation induced by CP and TEPA. METHODS Immunohistochemical methods were used to detect the protein expressions(IHC). RESULTS P16, P15, RB and cyclin D₁ were expressed in BEAS-2B cells, but not in BEAS-CP and BEAS-TE cells. P53 was expressed in BEAS-CP and BEAS- CE cells, but not in BEAS-2B cells. Cyclin D₁ was expressed in all the three cells. CONCLUSION Alterative expression of P16, P15 and RB proteins may directly lead to abnormality of the P16（P15）/cyclin D₁-CDK4/pRB pathway in cell cycle system of the two transformed cells, and positive expression of P53 may imply functional changes in P53 protein in both of transformed cells.